A cluster of hydrophobic amino acid residues required for fast Na(+)-channel inactivation.
نویسندگان
چکیده
منابع مشابه
A cluster of hydrophobic amino acid residues required for fast Na(+)-channel inactivation.
The inward Na+ current underlying the action potential in nerve is terminated by inactivation. The preceding report shows that deletions within the intracellular linker between domains III and IV remove inactivation, but mutation of conserved basic and paired acidic amino acids has little effect. Here we show that substitution of glutamine for three clustered hydrophobic amino acids, Ile-1488, ...
متن کاملAmino acid residues required for fast Na(+)-channel inactivation: charge neutralizations and deletions in the III-IV linker.
The cytoplasmic linker connecting domains III and IV of the voltage-gated Na+ channel is thought to be involved in fast inactivation. This linker is highly conserved among the various Na+ channels that have been cloned. In the rat brain IIA Na+ channel, it consists of 53 amino acids of which 15 are charged. To investigate the role of this linker in inactivation, we mutated all 15 of the charged...
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Fast Na+ channel inactivation is thought to involve binding of phenylalanine 1489 in the hydrophobic cluster IFM in L(III-IV) of the rat brain type IIA Na+ channel. We have analyzed macroscopic and single channel currents from Na+ channels with mutations within and adjacent to hydrophobic clusters in L(III-IV). Substitution of F1489 by a series of amino acids disrupted inactivation to different...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1992
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.89.22.10910