A Human Protein-Protein Interaction Network: A Resource for Annotating the Proteome
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Supplemental Data Resource A Human Protein-Protein Interaction Network: A Resource for Annotating the Proteome
Verification of Y2H Interactions by Coimmunoprecipitation and Pull-Down Assays To evaluate the quality of the Y2H data, a representative sample of Y2H interactions was randomly selected for verification assays, because interactions recapitulated independently are unlikely to be experimental false positives (Goehler et al., 2004 ̧ Tewari, 2004 #137). To verify Y2H interactions, we used in vitro p...
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Protein-protein interaction maps provide a valuable framework for a better understanding of the functional organization of the proteome. To detect interacting pairs of human proteins systematically, a protein matrix of 4456 baits and 5632 preys was screened by automated yeast two-hybrid (Y2H) interaction mating. We identified 3186 mostly novel interactions among 1705 proteins, resulting in a la...
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Background Oocyte maturity includes nuclear and cytoplasmic maturity, both of which are important for embryo fertilization. The development of oocyte is not limited to the period of follicular growth, and starts from the embryonic period and continues throughout life. In this study, for the purpose of evaluating the effect of the FSH hormone on the expression of genes, GEO access codes for this...
متن کاملAnnotating the human proteome.
The completion of the human genome has shifted the attention from deciphering the sequence to the identification and characterization of the encoded components. The identification and functional annotation of the proteome is here of special interest and starts with the identification of genes and transcripts as a prerequisite of proteome annotation. Gene predictions are very powerful in predict...
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ژورنال
عنوان ژورنال: Cell
سال: 2005
ISSN: 0092-8674
DOI: 10.1016/j.cell.2005.08.029