منابع مشابه
Transgenic mice: beyond the knockout.
Transgenic mice have had a tremendous impact on biomedical research. Most researchers are familiar with transgenic mice that carry Cre recombinase (Cre) and how they are used to create conditional knockouts. However, some researchers are less familiar with many of the other types of transgenic mice and their applications. For example, transgenic mice can be used to study biochemical and molecul...
متن کاملCharacterization of immunoglobulin heavy chain knockout rats.
The rat is a species frequently used in immunological studies but, until now, there were no models with introduced gene-specific mutations. In a recent study, we described for the first time the generation of novel rat lines with targeted mutations using zinc-finger nucleases. In this study, we compare immune development in two Ig heavy-chain KO lines; one with truncated Cμ and a new line with ...
متن کاملKnockout rats via embryo microinjection of zinc-finger nucleases.
The toolbox of rat genetics currently lacks the ability to introduce site-directed, heritable mutations into the genome to create knockout animals. By using engineered zinc-finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one-cell...
متن کاملAtherogenesis and metabolic dysregulation in LDL receptor-knockout rats.
Mechanisms of atherogenesis have been studied extensively in genetically engineered mice with disturbed cholesterol metabolism such as those lacking either the LDL receptor (Ldlr) or apolipoprotein E (apoe). Few other animal models of atherosclerosis are available. WT rabbits or rats, even on high-fat or high-cholesterol diets, develop sparse atherosclerotic lesions. We examined the effects of ...
متن کاملEfficient Simulation of a Random Knockout Tournament
We consider the problem of using simulation to efficiently estimate the win probabilities for participants in a general random knockout tournament. Both of our proposed estimators, one based on the notion of “observed survivals” and the other based on conditional expectation and post-stratification, are highly effective in terms of variance reduction when compared to the raw simulation estimato...
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ژورنال
عنوان ژورنال: Cell Cycle
سال: 2011
ISSN: 1538-4101,1551-4005
DOI: 10.4161/cc.10.7.15233