منابع مشابه
External Contamination in Single Cell mtDNA Analysis
BACKGROUND Mitochondrial DNA (mtDNA) variation in single hematopoietic cells, muscle fibers, oocytes, and from tiny amount of tumor tissues and degraded clinical specimens has been reported in many medical publications. External DNA contamination, notoriously difficult to avoid, threatens the integrity of such studies. METHODOLOGY/PRINCIPAL FINDINGS Employing a phylogenetic approach, we analy...
متن کاملAutomated Contamination Detection in Single-Cell Sequencing
Novel methods for the sequencing of single-cell DNA offer tremendous opportunities. However, many techniques are still in their infancy and a major obstacle is given by sample contamination with foreign DNA. In this contribution, we present a pipeline that allows for fast, automated detection of contaminated samples by the use of modern machine learning methods. First, a vectorial representatio...
متن کاملElimination of Mycoplasma Contamination in Cell Cultures
These antibiotic treatments had a high efficiency of permanent cure: MRA 64%, ciprofloxacin 77%, BM-Cyclin 84%. Resistance to mycoplasma eradication was observed in some cell cultures: BM-Cyclin 5%, ciprofloxacin 14%, MRA 22%. Nearly all resistant contaminants that could be identified belonged to the species M. arginini and M. orale. Detrimental effects of the antibiotics were seen in form of c...
متن کاملprevention and detection of mycoplasma contamination in cell culture
one of the main problems in cell culture is mycoplasma infection. it can extensively affect cell physiology and metabolism. as the applications of cell culture increase in research, industrial production and cell therapy, more concerns about mycoplasma contamination and detection will arise. this review will provide valuable information about: 1. the ways in which cells are contaminated and the...
متن کاملdetection of pestivirus contamination in cell cultures by nested-pcr
in this study a nested-pcr assay was optimized for detection of two bvdv biotype of nadl strain. a part of 5' non-coding region of virus, 249 bp in size, was amplified in rt-pcr. pcr product was cloned in a ptz57r/t vector and sequencing results confirmed the specificity of the test. internal primers were designed and a 155 bp dna fragment was amplified in nested-pcr. the sensitivity of rt...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature
سال: 1981
ISSN: 0028-0836,1476-4687
DOI: 10.1038/290008c0