Cell-to-Cell Transport of Movement-Defective Cucumber Mosaic and Tobacco Mosaic Viruses in Transgenic Plants Expressing Heterologous Movement Protein Genes
نویسندگان
چکیده
منابع مشابه
The cell biology of Tobacco mosaic virus replication and movement
Successful systemic infection of a plant by Tobacco mosaic virus (TMV) requires three processes that repeat over time: initial establishment and accumulation in invaded cells, intercellular movement, and systemic transport. Accumulation and intercellular movement of TMV necessarily involves intracellular transport by complexes containing virus and host proteins and virus RNA during a dynamic pr...
متن کاملMovement of a barley stripe mosaic virus chimera with a tobacco mosaic virus movement protein.
The tobacco mosaic virus (TMV) 30K movement protein (MP) gene was inserted into a full-length cDNA clone of barley stripe mosaic virus (BSMV) RNA beta replacing the triple gene block (TGB). The resulting recombinant ND-MPT genome, consisting of infectious wt transcripts of BSMV RNAs alpha and gamma, together with the hybrid RNA beta transcript, was inoculated onto test plants to study the funct...
متن کاملMimicking carboxyterminal phosphorylation differentially effects subcellular distribution and cell-to-cell movement of Tobacco mosaic virus movement protein.
Phosphorylation of Tobacco mosaic virus movement protein (TMV-MP) at three carboxyterminal Ser/Thr sites negatively regulates TMV-MP gating function and viral spread in Nicotiana tabacum but not in Nicotiana benthamiana, indicating a host dependant inactivation strategy. Here, we examine the effect of mimicking carboxyterminal phosphorylation on cell-to-cell transport of TMV-MP protein itself i...
متن کاملCharacterization of mutant tobacco mosaic virus coat protein that interferes with virus cell-to-cell movement.
Expression of tobacco mosaic virus (TMV) coat protein (CP) in plants confers resistance to infection by TMV and related tobamoviruses. Certain mutants of the CP (CP(T42W)) provide much greater levels of resistance than wild-type (wt) CP. In the present work, infection induced by RNA transcripts of TMV clones that contain wt CP or mutant CP(T42W) fused to the green fluorescent protein (GFP) (TMV...
متن کاملSalicylic acid has cell-specific effects on tobacco mosaic virus replication and cell-to-cell movement.
Tobacco mosaic virus (TMV) and Cucumber mosaic virus expressing green fluorescent protein (GFP) were used to probe the effects of salicylic acid (SA) on the cell biology of viral infection. Treatment of tobacco with SA restricted TMV.GFP to single-epidermal cell infection sites for at least 6 d post inoculation but did not affect infection sites of Cucumber mosaic virus expressing GFP. Microinj...
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ژورنال
عنوان ژورنال: Virology
سال: 1996
ISSN: 0042-6822
DOI: 10.1006/viro.1996.0048