Detection of Carbapenemases in Enterobacteriaceae by a Commercial Multiplex PCR


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Detection of carbapenemases in Enterobacteriaceae by a commercial multiplex PCR.

A commercial multiplex PCR (hyplex SuperBug ID) was tested with a collection of 132 clinical Enterobacteriaceae strains producing different carbapenemases. The sensitivity for the detection of KPC-, VIM-, NDM-, and OXA-48-encoding genes was 100%, whereas two IMP variants were missed.

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Primer Evaluation for PCR and its Application for Detection of Carbapenemases in Enterobacteriaceae

BACKGROUND During the last decade, the prevalence of carbapenem-resistant Enterobacteriaceae in human patients has increased. Carbapenemase-producing bacteria are usually multidrug resistant. Therefore, early recognition of carbapenemase producers is critical to prevent their spread. OBJECTIVES The objective of this study was to develop the primers for single and/or multiplex PCR amplificatio...

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عنوان ژورنال: Journal of Clinical Microbiology

سال: 2012

ISSN: 0095-1137

DOI: 10.1128/jcm.00991-12