Development of a new PCR-ribotyping method forClostridium difficilebased on ribosomal RNA gene sequencing
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چکیده
منابع مشابه
a sensitive and specific pcr based method for identification of cryptosporidium sp. using new primers from 18s ribosomal rna
background: the main goal of the present study was to develop a new sensitive and specific pcr based method for identification of cryptosporidium sp. using novel primers from 18s ribosomal rna. cryptosporidiosis in high-risk host groups particularly in neonates and immuno-compromised individuals may result in death. to the best of our knowledge this is the first study regarding develop a new p...
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One of the major questions in microbial ecology is "who is there?" This question can be answered using various tools, but one of the long-lasting gold standards is to sequence 16S ribosomal RNA (rRNA) gene amplicons generated by domain-level PCR reactions amplifying from genomic DNA. Traditionally, this was performed by cloning and Sanger (capillary electrophoresis) sequencing of PCR amplicons....
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Clostridium bacteraemia characterised by 16S ribosomal RNA gene sequencing.
BACKGROUND Owing to problems in accurate species identification of the diverse genus clostridium, the epidemiology and pathogenicity of many species are not fully understood. Moreover, previous studies on clostridium bacteraemia have been limited and relied only on phenotypic species identification. AIMS To characterise the epidemiology, disease spectrum, and outcome of clostridium bacteraemi...
متن کاملGlobicatella bacteraemia identified by 16S ribosomal RNA gene sequencing.
BACKGROUND Globicatella are streptococcus-like organisms that have been rarely isolated from clinical specimens. Their epidemiology and clinical significance remain largely unknown. AIMS To describe two cases of Globicatella bacteraemia identified by 16S ribosomal RNA (rRNA) gene sequencing. METHODS Two unidentified streptococcus-like bacteria isolated from blood cultures of patients were s...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1999
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1999.tb13629.x