Differential detection of vibrios pathogenic to shrimp by multiplex PCR
A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area...متن کامل
Detection of marine pathogenic bacterial Vibrio species by multiplex polymerase chain reaction (PCR).
Polymerase chain reactions (PCR) were performed to accomplish quick and accurate detection of Vibrio species. The primers prepared with 16S-23S rDNA intergenic spacer (IGS) region exhibited an excellent species-specificity for Vibrio sp and detected Vibrio sp more successfully than the conventional culture method. Multiplex PCR was also fruitful not only forthe identification of the 5 standard ...متن کامل
This study describes the development of a gene-specific DNA microarray coupled with multiplex PCR for the comprehensive detection of pathogenic vibrios that are natural inhabitants of warm coastal waters and shellfish. Multiplex PCR with vvh and viuB for Vibrio vulnificus, with ompU, toxR, tcpI, and hlyA for V. cholerae, and with tlh, tdh, trh, and open reading frame 8 for V. parahaemolyticus h...متن کامل
Simultaneous differential detection of human pathogenic and nonpathogenic Vibrio species using a multiplex PCR based on gyrB and pntA genes.
AIMS To develop a multiplex PCR targeting the gyrB and pntA genes for Vibrio species differentiation. METHODS AND RESULTS Four pairs of primers targeting gyrB gene of Vibrios at genus level and pntA gene of Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus were designed. This PCR method precisely identified 250 Vibrio species and demonstrated sensitivity in the range of 4 x 10(4) CF...متن کامل
Several species of helicobacter have been isolated from laboratory mice, including H. bilis, H. hepaticus, H. muridarum, H. rodentium, and H. typhlonius, which appear to be the most common. The most widely used published method for molecular detection of these agents is PCR amplification of a conserved region of 16S rRNA, but differential speciation requires restriction enzyme digestion of the ...متن کامل
عنوان ژورنال: The Journal of General and Applied Microbiology