EgyGene GelAnalyzer4: a powerful image analysis software for one-dimensional gel electrophoresis

Two-Dimensional Gel Electrophoresis Image Registration

Two-dimensional gel electrophoresis (2DGE) is the leading technique to separate individual proteins in biological samples with many biological and pharmaceutical applications, e.g., drug development. The technique results in an image in which the proteins appear as dark spots on a bright background. The analysis of these images is very time consuming and requires a large amount of manual work. ...

Multiresolution image registration for two-dimensional gel electrophoresis.

In proteomic research, two-dimensional electrophoresis (2-D) is an important tool for investigating differential patterns of qualitative and quantitative protein expression. The strength of the technique is due to its unrivalled power of being able to separate simultaneously thousands of proteins. The key to the comparison of 2-D protein profiles, however, lies in the use of a fast and robust i...

Comparative Evaluation of Tissue Protein Separations Applying One- Dimensional Gel Electrophoresis and Capillary Gel Electrophoresis

The objective of this study was to characterize and compare the water and salt soluble proteins from rat liver and muscle by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and sodium dodecyl sulphate polymer-filled capillary gel electrophoresis (SDS-CGE). The evaluation and comparison of separations realized with the use of both techniques was done. It was found tha...

Two-dimensional Gel Electrophoresis

Goldfish Brain GAP-43: Analysis by One and Two-Dimensional Gel Electrophoresis

Introduction Physiological and biochemical changes occurring in the brain following a learning task have mostly inferred through data stemming from the induced process of long-term potentiation (LTP). LTP is defined as a prolonged enhancement of following high frequency stimulation to fiber tracts in associative regions of brain (Bliss and Lomo, 1973; and Collingridge, 1993). Biological mechani...