Evaluation of Adding a Second Marker To Overcome Staphylococcus aureus spa Typing Homoplasies
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چکیده
منابع مشابه
Evaluation of adding a second marker to overcome Staphylococcus aureus spa typing homoplasies.
The utility of sequencing a second highly variable locus in addition to the spa gene (e.g., double-locus sequence typing [DLST]) was investigated to overcome limitations of a Staphylococcus aureus single-locus typing method. Although adding a second locus seemed to increase discriminatory power, it was not sufficient to definitively infer evolutionary relationships within a single multilocus se...
متن کاملspa Typing of Staphylococcus aureus as a frontline tool in epidemiological typing.
We determined the value of spa typing in combination with BURP (based upon repeat pattern) grouping analysis as a frontline tool in the epidemiological typing of Staphylococcus aureus, based on a random collection of 1,459 clinical isolates sent to the German Reference Centre for Staphylococci within a 6-month period. The application was found to be helpful for the classification of isolates in...
متن کاملEvaluation of spa-typing of methicillin-resistant Staphylococcus aureus using high-resolution melting analysis.
OBJECTIVE spa-typing of methicillin-resistant Staphylococcus aureus (MRSA) has been used widely in clinical diagnostics and epidemiological studies. The aim of this study was to evaluate high-resolution melting (HRM) as a rapid and cost-effective method, to replace DNA-sequencing, for spa-typing in a global collection of 50 MRSA isolates. METHODS The polymorphic X region of the spa gene was a...
متن کاملTransmission of Staphylococcus aureus from maternity unit staff members to newborns disclosed through spa typing.
BACKGROUND We observed previously that newborn infants are colonized with Staphylococcus aureus, even if their mothers do not carry S aureus. This observation indicated a cross colonization, and, thus, a risk for nosocomial infection, although the infants are roomed in with their mothers. METHODS The S aureus colonization of infants, their parents, and staff members was measured at 3 maternit...
متن کاملspa Typing and Multilocus Sequence Typing Show Comparable Performance in a Macroepidemiologic Study of Staphylococcus aureus in the United States
A number of molecular typing methods have been developed for characterization of Staphylococcus aureus isolates. The utility of these systems depends on the nature of the investigation for which they are used. We compared two commonly used methods of molecular typing, multilocus sequence typing (MLST) (and its clustering algorithm, Based Upon Related Sequence Type [BURST]) with the staphylococc...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2012
ISSN: 0095-1137
DOI: 10.1128/jcm.00664-11