Fabrication of anisotropic collagen-based substrates for potential use in tissue engineering

Abstract Stimuli-responsive nanomaterials have the prospective to enable fabrication of new extracellular matrix-like substrates with unique structures and cell-instructive capabilities. The development biocompatible collagen on-demand ordered architectures is an open challenge since it well-known that directionality fibres affects important cell behaviour, such as proliferation, differentiation, ultimately, tissue regeneration. Here, easy cheap approach fabricate anisotropic collagen-based exhibiting cells-instructing ability was proposed. Paramagnetic iron oxide nanoparticles (IOPs) coated polyethylene glycol were synthetized by a coprecipitation solvothermic method mixed precursors solution. suspension then immersed within static low-intensity magnetic field trigger IOPs self-assembly. Guided external stimulus, assembled along lines into long filamentous matrix. solidification pre-cursors solution in presence IOPs’ promotes organization fashions. obtained substrate demonstrated good cytocompatibility cells’ instructive properties.