Fs-laser-induced Ca^2+ concentration change during membrane perforation for cell transfection
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چکیده
منابع مشابه
Fs-laser-induced Ca2+ concentration change during membrane perforation for cell transfection.
Fs-laser based opto-perforation is a gentle method for gene transfer into sensitive cells such as stem cells or primary cells. The high selectivity and the low damage to the cell lead to a high efficiency of transfection. However, there are side effects which induce stress to the cell due to the exchange of intra- and extracellular media as well as the disintegration of the structure of biomole...
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Cells can be transfected by transient laser-induced perforation of the cell membrane which allows foreign genetic material to enter the cell interior. This method (“optoporation”) has emerged as a powerful noninvasive and highly efficient cell-transfection technique. A detailed comparison of efficiencies and results, however, is often hindered by different experimental setups and lasers used in...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2010
ISSN: 1094-4087
DOI: 10.1364/oe.18.002219