High affinity selenium uptake in a keratinocyte model

High-affinity sodium uptake in land plants.

High-affinity Na(+) uptake in plants and its mediation by HKT transporters have been studied in very few species. This study expands the knowledge of high-affinity Na(+) uptake in land plants for both uptake characteristics and involvement of HKT transporters. In non-flowering plants, we analyzed the Na(+) content of wild mosses, carried out experiments on K(+) and Na(+) uptake in the micromola...

High-affinity potassium uptake in plants.

oocyte]. Oocytes were incubated for 24 to 48 hours, treated with coelenterazine [10 ,uM coelenterazine (Molecular Probes, Eugene, OR) and 30 p.M reduced glutathione in OR-2 media (no calcium)] for 2 to 3 hours with gentle orbital shaking in the dark at 18°C, and retumed to ND-86 medium with calcium (maintained in the dark with shaking) until measurements were initiated. For luminometer measurem...

Effects of Different Rates of Selenium Fertilizer and Cow Manure on Selenium Uptake in Festuca arundinacea

One of the crucial elements for livestock and human nutrition is selenium and its deficiency or toxicity can harm human and livestock health. The present study is designed with the aim of studying the effect of different levels of selenium fertilizer (Selcote-Ultra) and cow manure on the uptake of selenium in tall fescue. For this purpose, a pot experiment with complete randomized block design ...

High-Affinity Potassium Uptake Transporter from Higher Plants

Reversible high affinity uptake of apo E-free high density lipoproteins in cultured pig hepatocytes.

We examined the high affinity binding, uptake, and degradation of apo E-free 125I-high density lipoprotein (HDL) in cultured pig hepatocytes. At steady state, the cells degraded 9.4% of cell-associated 125I-HDL/hour, compared with 41.7%/hour for 125I-LDL. Pulse-chase experiments at 4 degrees C revealed that high affinity 125I-HDL binding was reversible. Similar experiments at 37 degrees C revea...