Identification of a carbohydrate recognition domain in filamentous hemagglutinin from Bordetella pertussis
نویسندگان
چکیده
منابع مشابه
Subcutaneous administration of a fusion protein composed of pertussis toxin and filamentous hemagglutinin from Bordetella pertussis induces mucosal and systemic immune responses
Objective(s): After decades of containment, pertussis disease, caused by Bordetella pertussis seems to be re-emerging and still remains a major cause of reported vaccine-preventable deaths worldwide. The current licensed whole-cell vaccines display reactogenicity while acellular vaccines are expensive and do not induce Th1-type immune responses that are required for optimum protection against t...
متن کاملexpression, purification and characterization of three overlapping immunodominant recombinant fragments from bordetella pertussis filamentous hemagglutinin
background: filamentous hemagglutinin (fha) is one of the most important immunoprotective antigens of bordetella pertussis (b.pertussis) and a major component of the acellular pertussis vaccine. in the present study, three overlapping recombinant fragments from the immunodominant region of fha were produced and their immunogenicity was investigated. methods: three overlapping coding sequences o...
متن کاملExpression, Purification and Characterization of Three Overlapping Immunodominant Recombinant Fragments from Bordetella pertussis Filamentous Hemagglutinin
BACKGROUND Filamentous hemagglutinin (FHA) is one of the most important immunoprotective antigens of Bordetella pertussis (B. pertussis) and a major component of the acellular pertussis vaccine. In the present study, three overlapping recombinant fragments from the immunodominant region of FHA were produced and their immunogenicity was investigated. METHODS Three overlapping coding sequences ...
متن کاملAntigenic analysis of Bordetella pertussis filamentous hemagglutinin with phage display libraries and rabbit anti-filamentous hemagglutinin polyclonal antibodies.
Although substantial advancements have been made in the development of efficacious acellular vaccines against Bordetella pertussis, continued progress requires better understanding of the antigenic makeup of B. pertussis virulence factors, including filamentous hemagglutinin (FHA). To identify antigenic regions of FHA, phage display libraries constructed by using random fragments of the 10-kbp ...
متن کاملCharacterization of a recombinant fragment that contains a carbohydrate recognition domain of the filamentous hemagglutinin.
The filamentous hemagglutinin (FHA) of Bordetella pertussis plays an important role in establishing infection by attaching the bacteria to the ciliated respiratory epithelial cells. Expression of DNA encoding residues 1141 to 1279 of FHA in Escherichia coli yields a protein of 18,000 Da that exhibits some of the carbohydrate recognition properties of FHA (S. M. Prasad, Y. Yin, E. Rodzinski, E. ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1993
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.61.7.2780-2785.1993