Localization of Concanavalin A Binding Sites with a Concanavalin A-Alkaline Phosphatase Method
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چکیده
منابع مشابه
BINDING SITES ON CONCANAVALIN A 399 Concanavalin
Equilibrium dialysis of concanavalin A against methyl c¢-D-mannopyranoside and methyl C¢-D-glucopyranoside conducted at 2 ° in the presence of I M NaC1 showed that concanavalin A is bivalent. A SCATCHARD plot of the data obtained gave straight lines for both sugars with observed association constants (K') of 1. 4. lO 4 1/mole for methyl ,¢-D-mannopyranoside and o.3" IO 4 1/mole for methyl ~¢-D-...
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Plant lectins, because they bind to specific carbohydrate moieties, can be used to investigate the glyco-components of the cell surface. When considering the distribution, number, or mobility of lectin-binding sites, a distinction must be made between a natural distribution and a redistribution induced by binding with multivalent ligands (2, 4, 5, 8, 14, 15). In some cell types the induced redi...
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ژورنال
عنوان ژورنال: Okajimas Folia Anatomica Japonica
سال: 1979
ISSN: 0030-154X
DOI: 10.2535/ofaj1936.56.4_193