Multiplex PCR assay for discrimination of Centrocestus caninus and Stellantchasmus falcatus



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Susceptibility of rodents to Stellantchasmus falcatus infection.

Two rodent hosts, rats (Rattus norvegicus) and mice (Mus musculus), were examined for their susceptibility to Stellantchasmus falcatus infection. The metacercariae were recovered from half-beaked fish, Dermogenus pusillus, collected from Hang Dong District, Chiang Mai Province. A single inoculation of three hundred metacercariae was orally conducted with male rats (n=15) and mice (n=15). Uninfe...

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Some factors affecting Stellantchasmus falcatus metacercaria in laboratory.

Killing factors, various temperatures and solutions were studied in the laboratory on Stellantchasmus falcatus metacercaria in half-beaked fish (Dermogenus pusillus). Killing criteria followed the Movability Index from 1.000 within 24 hours. The metacercariae were collected from Chiang Mai moat. They were incubated in 0.85 % NaCl at -20 degrees C, room temperature, 4 degrees, 37 degrees, and 65...

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Molecular Markers for Identification of Stellantchasmus falcatus and a Phylogenic Study using the HAT-RAPD Method

Stellantchasmus falcatus is a minute intestinal fluke in the family Heterophyidae. Metacercariae, the infective stage, were reported in a marine fish, mullet Liza subviridis, and a fresh water fish, Dermogenus pusillus, in Thailand. Adults were found in chicks, rats, cats, and humans. Morphological studies were done for comparing Stellantchasmus sp. worms found in 2 different fish hosts; their ...

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Discrimination between E. granulosus sensu stricto, E. multilocularis and E. shiquicus Using a Multiplex PCR Assay

BACKGROUND Infections of Echinococcus granulosus sensu stricto (s.s), E. multilocularis and E. shiquicus are commonly found co-endemic on the Qinghai-Tibet plateau, China, and an efficient tool is needed to facilitate the detection of infected hosts and for species identification. METHODOLOGY/PRINCIPAL FINDINGS A single-tube multiplex PCR assay was established to differentiate the Echinococcu...

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MuPlex: multi-objective multiplex PCR assay design

We have developed a web-enabled system called MuPlex that aids researchers in the design of multiplex PCR assays. Multiplex PCR is a key technology for an endless list of applications, including detecting infectious microorganisms, whole-genome sequencing and closure, forensic analysis and for enabling flexible yet low-cost genotyping. However, the design of a multiplex PCR assays is computatio...

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عنوان ژورنال: Asian Pacific Journal of Tropical Biomedicine

سال: 2017

ISSN: 2221-1691

DOI: 10.1016/j.apjtb.2016.11.018