Neurofilament ELISA validation

Initial Validation of Cytokine Measurement by ELISA in Canine Feces

Measurement of fecal cytokines has been used as a marker of intestinal inflammation in people and correlates with endoscopic findings. The aim of this study was to evaluate the use of canine-specific enzyme-linked immunosorbant assays (ELISAs) for quantification of cytokines in canine fecal samples as a non-invasive biomarker. Interleukin (IL)-6, -8, -10, -23/12p40 and TNF- were assessed by us...

Validation of a Competitive ELISA for Detection of Babesia bigemina

Anti-signal recognition particle autoantibody ELISA validation and clinical associations.

OBJECTIVE The aim of this study was to develop and validate a quantitative anti-signal recognition particle (SRP) autoantibody serum ELISA in patients with myositis and longitudinal association with myositis disease activity. METHODS We developed a serum ELISA using recombinant purified full-length human SRP coated on ELISA plates and a secondary antibody that bound human IgG to detect anti-S...

Validation of an ELISA for the diagnosis of human trichinellosis

Validation of an ELISA for the diagnosis of human trichinellosis 1 2 Running title: Serodiagnosis of human trichinellosis 3 4 Maria Angeles Gómez-Morales, 1* Alessandra Ludovisi, 1 Marco Amati, 1 Simona Cherchi, 1 5 Patrizio Pezzotti, 2 and Edoardo Pozio 1 6 7 Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di 8 Sanità, 1 and Agenzia di Sanità Pubblica, 2 Rom...

Validation of a β-ME ELISA for Detection of Anti Leishmania donovani Antibodies in Eastern Sudan

Background: A β-mercaptoethnol (β-ME)-treated promastigote antigen of L. donovani was successfully employed in direct agglutination test (DAT) for the diagnosis of visceral leishmaniasis (VL). Objective: The β-ME-treated antigen was further incorporated into an enzyme-linked immunosorbent assay set-up (β-ME ELISA) and evaluated for VL diagnosis against outcome of reference freeze-dried DAT (FD-...