Observations on the system ascorbic acid-glutathione-ascorbic acid oxidase

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Clxxx. Further Observations on the System Ascorbic Acid-glutathione- Ascorbic Acid-oxidase by Eric Mitchell Crook and Frederick Gowland Hopkins

IN a paper by Hopkins & Morgan [1936] it was shown that in aqueous solutions containing Szent Gyorgyi's oxidase together with ascorbic acid and glutathione, the former is protected by the latter from oxidation by the enzyme, while the glutathione is itself oxidized vicariously at a rate corresponding to that at which the ascorbic acid is oxidized when alone with the enzyme in similar concentrat...

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Localization of ascorbic acid, ascorbic acid oxidase, and glutathione in roots of Cucurbita maxima L.

To understand the function of ascorbic acid (ASC) in root development, the distribution of ASC, ASC oxidase, and glutathione (GSH) were investigated in cells and tissues of the root apex of Cucubita maxima. ASC was regularly distributed in the cytosol of almost all root cells, with the exception of quiescent centre (QC) cells. ASC also occurred at the surface of the nuclear membrane and corresp...

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On the nature of ascorbic acid oxidase.

In 1937, Stotz, Harrer, and King (1) reported that the activity of several plant juices in catalyzing the aerobic oxidation of ascorbic acid was dependent on their copper content, and such activity was lost upon treatment with substances known to form stable complexes with copper. Since that time, two basically different views have developed concerning the r&e of copper in the aerobic oxidation...

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On the Inactivation of Ascorbic Acid Oxidase

1. In the absence of protective agents, highly purified ascorbic acid oxidase is rapidly inactivated during the enzymatic oxidation of ascorbic acid under optimum experimental conditions. This inactivation, called reaction inactivation to distinguish it from the loss in enzyme activity that frequently occurs in diluted solutions of the oxidase prior to the reaction, is indicated by incomplete o...

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Ascorbic Acid Oxidase in Citrus

1. Crall, J. M. and L. H. Stover, 1957. The significance of Pierce's disease in the decline of bunch grapes in Florida. lAbst.) Phytopath. 47: 518. 2. Dickey, R. D. and K. W. Loucks, (revised by Dickey, R. D., L. H. Stover, and G. K. Parris) 1947. Grape growing, in Florida. Fla. Agr. Exp. Sta. Bui. 436 3. Dubois, C. W. Personal communication. 4. Pederson, Carl S., 1961. Grape juice. Chapter 23 ...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1938

ISSN: 0306-3283

DOI: 10.1042/bj0320621