Oxidation of 7-dehydrocholesterol and desmosterol by human cytochrome P450 46A1
نویسندگان
چکیده
منابع مشابه
In vitro cytochrome P450 46A1 (CYP46A1) activation by neuroactive compounds.
Cytochrome P450 46A1 (CYP46A1, cholesterol 24-hydroxylase) is the enzyme responsible for the majority of cholesterol elimination from the brain. Previously, we found that the anti-HIV drug efavirenz (EFV) can pharmacologically activate CYP46A1 in mice. Herein, we investigated whether CYP46A1 could also be activated by endogenous compounds, including major neurotransmitters. In vitro experiments...
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Human cytochrome P450 (P450) 1B1 (CYP1B1) has recently been shown to be an important enzyme in the activation of diverse procarcinogens such as arylarenes, nitroarenes, and arylamines to reactive metabolites that cause DNA damage in the cells. However, it is not known whether this P450 enzyme also plays roles in the oxidation of certain drugs or model substrates commonly used in P450 assays. We...
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Cytochrome P450 (P450) 2D6 is involved in the oxidation of a large fraction ( 30%) of drugs used by humans and also catalyzes the O-demethylation of the model substrates 3and 4-methoxyphenethylamine followed by subsequent ring hydroxylation to dopamine. Burst kinetics were not observed; rate-limiting step(s) must occur prior to product formation. Rates of reduction of ferric P450 2D6 were stimu...
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Estrogen and its metabolites are believed to play important roles in breast cancer. The influence of genetic polymorphisms in the enzymes responsible for formation and disposition of estrogen on breast cancer risk may shed light on the importance of estrogen metabolites in this disease. However, for such studies to be valid, it is important to correctly identify the enzymes involved in estrogen...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 2014
ISSN: 0022-2275
DOI: 10.1194/jlr.m051508