PREPARATION OF P 32 -LABELED COXIELLA BURNETII CELLS
نویسندگان
چکیده
منابع مشابه
Coxiella burnetii
Q fever has been described for the first time in 1935 as an outbreak of fever in workers of a slaughterhouse in Brisbane, Australia (Derrick, 1937). Derrick could not identify the aetiological agent therefore he defined the disease as “query fever”. Afterwards, in 1937, Burnet and Freeman (Burnet & Freeman, 1937) isolated a troublesome intracellular pathogen from mice inoculated with blood or f...
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Coxiella burnetii was isolated from persistently infected fibroblast host cells by a rapid mechanical lysis technique. Macromolecular synthesis was initiated in these otherwise dormant cells by incubation at pH 4.5. The synthesis of protein proceeded for as long as 24 h. Initiation of protein synthesis in C. burnetii was dependent upon RNA synthesis. Approximately 24 species of polypeptides wer...
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This report reviews the pulmonary and extrapulmonary manifestation of infections due to Coxiella burnetii. Q fever, a zoonosis, is due to infection with C. burnetii. This spore-forming microorganism is a small gram-negative coccobacillus that is an obligate intracellular parasite. The most common animal reservoirs are goats, cattle, sheep, cats, and occasionally dogs. The organism reaches high ...
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Coxiella burnetii has the potential to cause serious disease and is highly prevalent in the environment. Despite this, epidemiological data are sparse and isolate collections are typically small, rare, and difficult to share among laboratories as this pathogen is governed by select agent rules and fastidious to culture. With the advent of whole genome sequencing, some of this knowledge gap has ...
متن کاملCoxiella burnetii Genotyping
Coxiella burnetii is a strict intracellular bacterium with potential as a bioterrorism agent. To characterize different isolates of C. burnetii at the molecular level, we performed multispacer sequence typing (MST). MST is based on intergenic region sequencing. These regions are potentially variable since they are subject to lower selection pressure than the adjacent genes. We screened 68 space...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1962
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.84.4.873-873.1962