منابع مشابه
Analysis of primer-derived, nonspecific amplification products in RAPD-PCR.
Recently, we applied the randomamplified polymorphic DNA polymerase chain reaction (RAPD-PCR) (17), also known as arbitrarily primed PCR (AP-PCR) (16), procedure to fingerprint genomes of various varieties and wild relatives of sugarcane (Saccharum spp.) (4). Unlike restriction fragment length polymorphism (RFLP) (2), RAPD-PCR is a simpler, faster, less laborious and less expensive procedure. H...
متن کاملA Simple Genome Walking Strategy to Isolate Unknown Genomic Regions Using Long Primer and RAPD Primer
Background: Genome walking is a DNA-cloning methodology that is used to isolate unknown genomic regions adjacent to known sequences. However, the existing genome-walking methods have their own limitations. Objectives: Our aim was to provide a simple and efficient genome-walking technology. Material and Methods: In this paper, we dev...
متن کاملElemental analysis of primer mixtures and gunshot residues from handgun cartridges commonly encountered in Taiwan
Inorganic gunshot residues (GSR) can arise from each component of the fired cartridge and GSR deposits inside the barrel from previous shootings. This work focused on the analysis of primer mixtures that yield GSR-unique particles. Primer mixtures and gunshot residues from 25 kinds of cartridges were recovered and subjected to scanning electron microscopy/ energy dispersive X-ray analysis to ob...
متن کاملParentage analysis using RAPD PCR.
DNA variation from randomly amplified polymorphic DNA (RAPD) (1, 2) has recently been helpful in addressing a wide range of evolutionary problems including the determination of paternity and maternity (3). It has been greeted with particular enthusiasm because it is fast, technically easy and requires little material (4). By using single 10 base primers of arbitrary sequences to amplify DNA wit...
متن کاملRAPD Analysis for Sex Determination in Pistacia vera L.
Random Amplified Polymorphic DNA (RAPD) analysis of Pistacia vera L. was used to distinguishing sex type in progeny plants. Varied genotypes of the pistachio were analyzed by PCR amplification of a ten base primer previously found to be linked to the expression of a 945bp amplification band in female trees and its absence in males. Results revealed that genetic material from cultivar samples am...
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ژورنال
عنوان ژورنال: Hereditas
سال: 2004
ISSN: 0018-0661
DOI: 10.1111/j.1601-5223.2000.00203.x