QTL Mapping of Leaf-Related Traits Using a High-Density Bin Map in Brassica rapa

The species Brassica rapa includes enormous leafy vegetables with extreme leaf morphological diversity. Leaf traits such as size, shape, weight, and ratio of the blade to petiole contribute yield, appearance, desirability consumers. These leaf-related are controlled by quantitative trait loci (QTLs). construction high-density bin maps using low-coverage sequencing is a powerful method for QTL fine-mapping gene identification. In this study, we performed whole-genome re-sequencing Wutacai ‘Zhongbaye’ Chinese cabbage ‘HN53’ 150 F2 individuals construct map mapping 11 traits. parental lines population were re-sequenced at 10x 1x coverage, respectively. A containing 565 markers was constructed based on single-nucleotide polymorphisms modified sliding window approach. total length 944.6 cM average distance bins 1.65 cM. total, 60 significant QTLs controlling detected. We further identified candidate genes responsible these complex findings suggest that cost-effective bin-mapping approach capable rapid identification genes, will thus facilitate dissection underlying molecular basis variations accelerate improvement B. vegetable breeding.