Simultaneous quantification of several mRNA species by solution hybridisation with oligonucleotides
نویسندگان
چکیده
منابع مشابه
Detection of several mRNA species in rice phloem sap.
Evidence is reported for the presence of the mRNAs of thioredoxin h, oryzacystatin-I, and actin in the rice phloem sap collected by the insect laser method. As the sieve element, the core component of the phloem, is enucleated, these macromolecules are probably transported from the companion cells.
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Simultaneous in situ hybridisation of native mRNA and immunoglobulin detection by conventional immunofluorescence in paraffin wax embedded sections.
AIMS The development of a technique for simultaneous in situ hybridisation for native mRNA and conventional immunofluorescence for cytoplasmic antigens in routine pathology specimens. METHODS Cocktails of synthetic deoxyoligonucleotides coding for immunoglobulin J chain and kappa light chain were 3' end labelled enzymatically with digoxigenin using terminal deoxynucleotidyl transferase. Nativ...
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BACKGROUND Currently, comparative genomic hybridisation array (array CGH) is the method of choice for studying genome wide DNA copy number changes. To date, either amplified representations of bacterial artificial chromosomes (BACs)/phage artificial chromosomes (PACs) or cDNAs have been spotted as probes. The production of BAC/PAC and cDNA arrays is time consuming and expensive. AIM To evalua...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.12.3466