investigating the cytotoxic effects of persian gulf marine actinomycetes protease on blood cancer cell line

نویسندگان

zeinab bandari m.sc. department of microbiology, school of basic sciences, islamic azad university (science and research unit), fars, iran

elham mozamian assistant professor, department of microbiology, school of science, agriculture and novel technologies, shiraz unit, islamic azad university, shiraz, iran

negar azarpira assistant professor, professor alborzi center for clinical microbiology research, shiraz university of medical sciences, shiraz, iran

چکیده

background: considering their specific adaption to marine milieus, marine actinomycetes constitute an excellent source for producing unique bioactive substances. they are also important sources of antibiotics and extracellular enzymes, geosmin, and enzymes with potent antibacterial and anticarcinogenic properties and low toxicity. the objective of this study is to isolate actinomycetes producing antitumor substances from the sediments of hara forests in the persian gulf and investigate the positive in vitro effects of these microorganisms through protease production on blood cancer cell lines. methods: in this study, 30 samples of sediments were collected from hara forests. after the strains were isolated, the samples were identified using morphologic characteristics. the protease was extracted and its cytocidal activity on the aacute lymphoblastic leukemia (all) was studied. results: the results indicate that out of the 30 actinomycetal isolates, 15 specimens were capable of producing protease, of which 2 affected the malignant cell line. in addition, statistical analysis revealed that the protease exerted greater cytocidal effect on the blood cancer cell line at higher concentrations. the cytopathic property of the protease was observed as cell rupture. conclusions: our findings indicate that sediments of the hara forests in the persian gulf are rich in active actinomycetes producing novel anticarcinogenic substances, warranting identification and purification. these results suggest the need for studies on marine microbes as a new potential for pharmaceutical research.

برای دسترسی به متن کامل این مقاله و 23 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Investigating the effects of Sea anemone (Stichodactyla haddoni) mucus on prostate cancer PC3 cell line

Background & Aim: Prostate cancer (PCa) is one of the most common cancers of the male urinary system and is also the second leading cause of cancer deaths in men. In the present study, the effects of Stichodactyla haddoni on the viability of prostate cancer cells (PC3) were investigated. Methods: In the semi experimental study, sea anemones were sampled at random using an identification key. T...

متن کامل

Cytotoxic Effects of Aqueous Extract of Portulaca oleracea on Oral Cancer Cell Line

Background: Portulaca oleracea as a herbal plant has been widely used in traditional medicine to cure many diseases. The therapeutic effects of this plant are exerted through its active ingredients with antioxidant properties. It has been shown that this plant may have cytotoxic effects on cancer cells. In the present study, cytotoxic effects of aqueous extract of Portulaca oleracea on oral cav...

متن کامل

Chitosan extracted from the Persian Gulf chiton shells: Induction of apoptosis in liver cancer cell line

Here for the first time, we investigated the cytotoxic effects of the chitosan extracted from the Persian Gulf Chiton shell (Acanthopleura vaillantii) on liver cancer cell line (HepG2). Chitosan extraction was implemented following this method: chitin was produced by demineralization and deproteinization procedure, and the extracted chitin was converted into soluble chitosan using deacetylation...

متن کامل

The Anti-cancer effects of Celastrol on K562 cell line

  Background and Objective: The level of NF-κB factor expression (a transcriptional factor which increases the expression of inflammatory genes) is often increased in various human cancers. Therefore, NF-κB inhibitors such as Celastrol may prevent cancer development. The purpose of this study was to evaluate the anticancer effects of Celastrol on K562 cells proliferation. Materials and Methods:...

متن کامل

Chitosan extracted from the Persian Gulf chiton shells: Induction of apoptosis in liver cancer cell line

Here for the first time, we investigated the cytotoxic effects of the chitosan extracted from the Persian Gulf Chiton shell (Acanthopleura vaillantii) on liver cancer cell line (HepG2). Chitosan extraction was implemented following this method: chitin was produced by demineralization and deproteinization procedure, and the extracted chitin was converted into soluble chitosan using deacetylation...

متن کامل

Molecular Identification, Isolation and Evaluation of Persian Gulf Actinomycetes as Candidates of Cytotoxic Metabolites Against Breast Cancer

Introduction: Marine actinomycetes have a great potential to produce unique bioactive compounds due to their special adaptation in the harsh ocean environment. The current study aimed to isolate anti-cancer compounds producing actinomycetes from sediments of Harra forests of the Persian Gulf and investigate their potential as anti-breast cancer metabolites. Methods: In the current study, 40 se...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

download full

برای دسترسی به متن کامل این مقاله و 23 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
basic and clinical cancer research

جلد ۸، شماره ۴، صفحات ۳-۱۴

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2022