increased litter size and suckling intensity inhibit kiss-1 mrna expression in rat arcuate nucleus
نویسندگان
چکیده
objective(s): the effect of litter size and suckling intensity on the expression of kiss-1 mrna in the arcuate nucleus (arc) of rats were evaluated. materials and methods: thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. lactating rats were allotted to eight equal groups. in three groups, litter size was adjusted to 5, 10, or 15 pups upon parturition and allowed to suckle their pups continuously by 8 days postpartum. in the other three groups, litter size was adjusted to five upon birth; the pups were separated from the dams for 6 hr on day 8 postpartum, after which the pups were allowed to suckle their dams for 2.5, 5, or 7.5 min prior to killing the dams. two groups of lactating rats with either 10 or 15 pups were separated from their pups for 6 hr on day 8 postpartum, after which the pups were allowed to suckle their dams for 5 min before the dams were killed on day 8 postpartum. the arc was removed and the expression of kiss-1 mrna was evaluated, using real-time pcr. results: the expression of kiss-1 mrna in the arc was decreased as the litter size and intensity of suckling stimulus were increased. the effect of suckling intensity on the expression of kiss-1 mrna was more pronounced than that of litter size. conclusion: increased litter size and suckling intensity decreased kiss-1 mrna expression in the arc which may contribute to lactation anestrus in rat.
منابع مشابه
Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus
Objective(s): The effect of litter size and suckling intensity on the expression of KiSS-1 mRNA in the arcuate nucleus (ARC) of rats were evaluated. Materials and Methods: Thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. Lactating rats were allotted to eight equal groups. In three groups, litter size was adjusted to 5, 10, or 15 pup...
متن کاملIncreased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus
OBJECTIVES The effect of litter size and suckling intensity on the expression of KiSS-1 mRNA in the arcuate nucleus (ARC) of rats were evaluated. MATERIALS AND METHODS Thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. Lactating rats were allotted to eight equal groups. In three groups, litter size was adjusted to 5, 10, or 15 pups ...
متن کاملIncreased Litter Size and Suckling Intensity Stimulate mRNA of RFamide-related Peptide in Rats
Background RFamide-related peptide-3 (RFRP-3) inhibits gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in rats. This study evaluates the effects of litter size and suckling intensity on RFRP mRNA expression in the dorsomedial hypothalamic nucleus (DMH) of rats. MaterialsAndMethods A total of 32 pregnant and 4 non-lactating ovariectomized (control group) Sprague-Dawl...
متن کاملExpression of RFamide-Related Peptide-3 (RFRP-3) mRNA in Dorsomedial Hypothalamic Nucleus and KiSS-1 mRNA in Arcuate Nucleus of Rat during Pregnancy
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P-5: Effect of Chronic Stress on Kiss-1 mRNA Expression in Male Rat Arcuate Hypothalamic Nucleus
Background Kisspeptin is a potent stimulator of the hypothalamic- pituitary-gonadal axis and may play a critical role in modulating of stress-induced suppression of reproduction. The aim of the present study was to investigate the effect of glucocorticoid receptor antagonist (RU486) and stress on expression of Kiss-1 mRNA in arcuate nucleus (ARC) of rat hypothalamus. MaterialsAndMethods Twenty ...
متن کاملincreased litter size and suckling intensity stimulate mrna of rfamide-related peptide in rats
background: rfamide-related peptide-3 (rfrp-3) inhibits gonadotropin releasing hormone (gnrh) and luteinizing hormone (lh) secretion in rats. this study evaluates the effects of litter size and suckling intensity on rfrp mrna expression in the dorsomedial hypothalamic nucleus (dmh) of rats. materials and methods: a total of 32 pregnant and 4 non-lactating ovariectomized (control group) sprague-...
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عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۱۷، شماره ۸، صفحات ۶۰۰-۶۰۵
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