computational prediction of the effects of single nucleotide polymorphisms of the gene encoding human endothelial nitric oxide synthase
نویسندگان
چکیده
abstract introduction: genetic variations in the gene encoding endothelial nitric oxide synthase (enos) enzyme affect the susceptibility to cardiovascular disease. identification of the way these changes affect enos structure and function in laboratory conditions is difficult and time-consuming. thus, it seems essential to perform bioinformatics studies prior to laboratory studies to find the variants that are more important. this study aimed to predict the damaging effect of changes in the coding region of enos using homology- and structure-based algorithms (sift and polyphen). methods: first, the single nucleotide polymorphisms in the coding region (csnps) of the human enos gene were extracted from dbsnp. resulting amino acid changes were reported as primary data required for the study. then, position and type of amino acid changes along with the complete amino acid sequence were separately entered into the sift and polyphen tools for analysis. results: of 144 single nucleotide changes, 38 changes by the sift, 47 changes by the polyphen and 18 amino acid substitutions by both tools were predicted as damaging. conclusion: it is predicted that 18 amino acid changes may have damaging phenotypic effects on the structure of the enos enzyme that may affect its performance by potentially affecting the enzyme’s various functional regions. therefore, computational prediction of potentially damaging nssnps and prioritizing amino acid changes may be useful for investigating protein performance using targeted re-sequencing and gene mutagenesis experiments.
منابع مشابه
Computational Prediction of the Effects of Single Nucleotide Polymorphisms of the Gene Encoding Human Endothelial Nitric Oxide Synthase
ABSTRACT Background and Objective: Genetic variations in the gene encoding endothelial nitric oxide synthase (eNOS) enzyme affect the susceptibility to cardiovascular disease. Identification of the way these changes affect eNOS structure and function in laboratory conditions is difficult and time-consuming. Thus, it seems essential to ...
متن کاملEXPRESSION OF INDUCIBLE NITRIC OXIDE SYNTHASE GENE (iNOS) STIMULATED BY HYDROGEN PEROXIDE IN HUMAN ENDOTHELIAL CELLS
Inducible nitric oxide synthase (iNOS) gene expresses a calcium calmudolin-independent enzyme which can catalyse NO production from L-arginine. The induction of iNOS activity has been demonstrated in a wide variety of cell types under stimulation with cytokines and lipopoly saccharide (LPS). Previous studies indicated that all nitric oxide synthases (NOS) activated in human umbilical vein endot...
متن کاملthe association of endothelial nitric oxide synthase gene polymorphisms and preeclampsia susceptibility
متن کامل
the effects of planning on accuracy and complexity of iranian efl students’ written narrative task performance
this study compared the different effects of form-focused guided planning vs. meaning-focused guided planning on iranian pre-intermediate students’ task performance. the study lasted for three weeks and concentrated on eight english structures. forty five pre-intermediate iranian students were randomly assigned to three groups of guided planning focus-on-form group (gpfg), guided planning focus...
15 صفحه اولthe effect of taftan pozzolan on the compressive strength of concrete in the environmental conditions of oman sea (chabahar port)
cement is an essential ingredient in the concrete buildings. for production of cement considerable amount of fossil fuel and electrical energy is consumed. on the other hand for generating one tone of portland cement, nearly one ton of carbon dioxide is released. it shows that 7 percent of the total released carbon dioxide in the world relates to the cement industry. considering ecological issu...
منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
medical laboratory journalجلد ۱۰، شماره ۳، صفحات ۱-۵
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023