comparison of differentiation potential of male mouse adipose tissue and bone marrow derived-mesenchymal stem cells into germ cells

نویسندگان

maryam hosseinzadeh shirzeily

parichehr pasbakhsh

fardin amidi

kobra mehrannia

چکیده

background: recent publications about differentiation of stem cells to germ cells have motivated researchers to make new approaches to infertility. in vitro production of germ cells improves understanding differentiation process of male and female germ cells. due to the problem of using embryonic stem cells (esc), it’s necessary the mentioned cells be replaced with some adult multi-potent stem cells in laboratories. objective: the aim of this study was to obtain germ cells from appropriate source beyond esc and compare differential potentials of adipocytes derived stem cells (admscs) with bone marrow derived stem cells (bmmscs). materials and methods: to find multi-potential entity, after providing purified admscs and bmmscs, differentiation to osteoblast and adipocyte was confirmed by using appropriate culture medium. to confirm mesenchymal lineage production superficial markers (expression of cd90 and cd44 and non-expression of cd45 and cd31) were investigated by flowcytometry. then the cells were differentiated to germ cells in inductive medium containing retinoic acid for 7days. to evaluate germ cells characteristic markers [dazl (deleted in azoospermia-like), mvh (mouse vasa homolog gene), stra8 (stimulated by retinoic acid) and scp3 (synaptonemal complex protein 3)] flowcytometry, imunoflorescence and real time pcr were used. results: both types of cells were able to differentiate into osteoblast and adipocyte cells and presentation of stem cell superficial markers (cd90, cd44) and absence of endothelial and blood cell markers (cd31, cd45) were confirmative the flowcytometry, imunoflorescence and real time pcr results showed remarkable expression of germ cells characteristic markers (mvh, dazl, stra8, and scp3). conclusion: it was found that although admscs were attained easier and also cultured and differentiated rapidly, germ cell markers were expressed in bmmscs significantly more than admscs.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Comparison of differentiation potential of male mouse adipose tissue and bone marrow derived-mesenchymal stem cells into germ cells

BACKGROUND Recent publications about differentiation of stem cells to germ cells have motivated researchers to make new approaches to infertility. In vitro production of germ cells improves understanding differentiation process of male and female germ cells. Due to the problem of using embryonic stem cells (ESC), it's necessary the mentioned cells be replaced with some adult multi-potent stem c...

متن کامل

Differentiation Potential of Nestin (+) and Nestin (-) Cells Derived from Human Bone Marrow Mesenchymal Stem Cells into Functional Insulin Producing Cells

The feasibility of isolating and manipulating mesenchymal stem cells (MSCs) from human patients provides hope for curing numerous disease and disorders. Recent phenotypic analysis showed heterogeneity of MSCs. A nestin progenitor cell is a subpopulation within MSCs which plays a role in pancreas regeneration during embryogenesis. This study aimed to separate nestin (+) cells from human bone mar...

متن کامل

Differentiation of Adipose-derived Stem Cells into Schwann Cell Phenotype in Comparison with Bone Marrow Stem Cells

Objective(s) Bone marrow is the traditional source of human multipotent mesenchymal stem cells (MSCs), but adipose tissue appears to be an alternative and more readily available source. In this study, rat adipose-derived stem cells (ADSCs) were induced to differentiate into Schwann-like cells and compared with rat bone marrow stem cells (BMSCs) for their Schwann-like cells differentiation pote...

متن کامل

comparison of proliferative and multilineage differentiation potential of sheep mesenchymal stem cells derived from bone marrow, liver, and adipose tissue

background: despite major progress in our general knowledge related to the application of adult stem cells, finding alternative sources for bone marrow mesenchymal stem cells (mscs) has remained to be challenged. in this study successful isolation, multilineage differentiation, and proliferation potentials of sheep mscs derived from bone marrow, adipose tissue, and liver were widely investigate...

متن کامل

Osteogenic Differentiation of Rat Mesenchymal Stem Cells from Adipose Tissue in Comparison with Bone Marrow Mesenchymal Stem Cells: Melatonin As a Differentiation Factor

Background: Adipose-derived stem cells (ADSC) could be an appealing alternative to bone marrow stem cells (BMSC) for engineering cell-based osteoinductive grafts. Meanwhile, prior studies have demonstrated that melatonin can stimulate osteogenic differentiation. Therefore, we assayed and compared the melatonin effect on osteogenic differentiation of BMSC with that of ADSC. Methods: Mesenchymal...

متن کامل

Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells into Insulin Producing Cells Using Minimal Differentiation Factors

Background & Aims: Type 1 diabetes, or insulin-dependent diabetes, is an autoimmune disease in which pancreatic beta cells are destroyed by the immune system. Hitherto, no definite treatment has been found for this condition. Mesenchymal stem cells (MSCs) are multipotent, self-renewing cells that have the ability to differentiate into mesodermal tissues. This ability has attracted the attention...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of reproductive biomedicine

جلد ۱۱، شماره ۱۲، صفحات ۹۶۵-۰

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023