High-pressure liquid chromatography: separation

نویسندگان

  • Glenville Jones
  • Hector F. DeLuca
چکیده

The high-pressure liquid chromatographic separation of ail of the known metabolites of vitamin D2 and vitamin D3 found in biological fluids has been achieved. This technique has been successfully applied to the analysis of vitamin D mixtures, purification of vitamin D metabolites, and identification of radioactive peaks. Some theoretical bases for the observed resolutions are suggested. Supplementary key words 1,25-dihydroxyvitamin D3 25-hydroxyvi-

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Separation of Sedative – Hypnotic Drugs with Mixed Micellar Liquid Chromatography

Separation of ten sedative- hypnotic drugs was performed by RP-HPLC using mixed micellar mobile phase. Effect of temperature, type and amount of organic modifier in mobile phase on efficiency (N) and asymmetry factor (B/A) showed that, the appropriate conditions for a good separation were 35°C and 7% (V/V) butanol in mobile phase.  Variations of selectivity factor versus butanol concent...

متن کامل

Separation of Aromatic and Alcoholic Mixtures using Novel MWCNT-Silica Gel Nanocomposite as an Adsorbent in Gas Chromatography

The separations of alcohols with hydrophilic and hydrophobic parts, and the separation of aromatic mixtures, are extremely important processes in gas and petroleum industries. Choosing an adsorbent for performing this separation is the most important part of the process. Silica gel is used as an adsorbent is various techniques such as pressure swing adsorption (PSA) and gas and liquid chromatog...

متن کامل

Hplc Separation of Phospholipids

The principles involved in the selection of solvents and the use of counter ions, together with the choice of column, for the separation of phospholipids by highperformance liquid chromatography (HPLC) are described.

متن کامل

Separation, identification, and quantification of carotenoids in fruits, vegetables and human plasma by high performance liquid chromatography

Separation, identification, and quantitation by high performance liquid chromatography of prominent carotenoids and carotenol fatty acid esters in various fruits and vegetables are reviewed. Examples of transformations of carotenoids as a result of food preparation are discussed. Recent developments in separation and identification of several carotenoids in human plasma are presented.

متن کامل

Quantification of identical and unique segments in ethylene-propylene copolymers using two dimensional liquid chromatography with infra-red detection

Hyphenating High Temperature High Performance Liquid Chromatography (HT-HPLC) with High Temperature Size Exclusion Chromatography (HT-SEC) (High Temperature Two Dimensional Liquid Chromatography (HT-HPLC x HT-SEC or HT 2D-LC)) leads to an isocratic elution in the second dimension, which in turn enables to use IR detector (quantitative detection) for monitoring the eluting polymers. Experimental...

متن کامل

High pressure liquid chromatography solvent systems for studies of bile acid biosynthesis.

Reversed phase high pressure liquid chromatography (HPLC) solvent systems have been developed for the separation of intermediates in the formation of bile acids and bile acid conjugates from cholesterol. Four different mobile phases (water-methanol, 10 mM acetate buffer (pH 4.37)-methanol, 30 mM trifluoroacetic acid (pH 2.9 with triethylamine)-methanol, and 50 mM potassium phosphate buffer (pH ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2002