Facile functionalization of a fully fluorescent perfluorophenyl BODIPY: photostable thiol and amine conjugates.

نویسندگان

  • Guillaume Vives
  • Carlo Giansante
  • Robin Bofinger
  • Guillaume Raffy
  • André Del Guerzo
  • Brice Kauffmann
  • Pinar Batat
  • Gediminas Jonusauskas
  • Nathan D McClenaghan
چکیده

Selective nucleophilic substitution on a perfluorophenyl unit comprising a BODIPY fluorophore using an alkanethiol or a primary amine offers a quantitative fluorophore labelling strategy, while retaining high photostability and emission quantum yields approaching unity.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Section 11.1 - Probes for Actin

Fluorescently labeled actin (Figure 11.1) is an important tool for investigating cytoskeleton dynamics in vivo.1–5 Molecular Probes offers highly purified actin from rabbit muscle (A-12375), as well as fluorescent actin conjugates labeled with two of our brightest and most photostable dyes. The green-fluorescent Alexa Fluor 488 actin conjugate (A-12373) has excitation and emission maxima simila...

متن کامل

Facile Preparation of Fluorescent Neoglycoproteins Using p-Nitrophenyl Anthranilate as a Heterobifunctional Linker

A facile preparation of neoglycoconjugates has been developed with a commercially available chemical, p-nitrophenyl anthranilate (PNPA), as a heterobifunctional linker. The two functional groups of PNPA, the aromatic amine and the p-nitrophenyl ester, are fully differentiated to selectively conjugate with glycans and other biomolecules containing nucleophiles. PNPA is efficiently conjugated wit...

متن کامل

Bio-orthogonal phosphatidylserine conjugates for delivery and imaging applications.

The syntheses of phosphatidylserine (PS) conjugates are described, including fluorescent derivatives for potential cellular delivery and bioimaging applications. Installation of terminal functional groups (amine, thiol, or alkyne) onto the sn-2 chain provides reactive sites for bio-orthogonal conjugation of cargo with suitably protected PS derivatives. An amine-containing PS forms amide bonds w...

متن کامل

Covalent Functionalization of GaP(110) Surfaces via a Staudinger- Type Reaction with Perfluorophenyl Azide

Despite the markedly low chemical reactivity of the nonpolar (110) surfaces of III−V semiconductors, the covalent functionalization of GaP(110) surfaces with perfluorophenyl azide (PFPA) molecules by a Staudinger-type reaction occurs only slightly above room temperature (325 K). Scanning tunneling microscopy observations, combined with density functional theory calculations, support the formati...

متن کامل

Facile functionalization of polyesters through thiol-yne chemistry for the design of degradable, cell-penetrating and gene delivery dual-functional agents.

Synthesis of polyesters bearing pendant amine groups with controlled molecular weights and narrow molecular weight distributions was achieved through ring-opening polymerization of 5-(4-(prop-2-yn-1-yloxy)benzyl)-1,3-dioxolane-2,4-dione, an O-carboxyanhydride derived from tyrosine, followed by thiol-yne "click" photochemistry with 2-aminoethanethiol hydrochloride. This class of biodegradable po...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Chemical communications

دوره 47 37  شماره 

صفحات  -

تاریخ انتشار 2011