High Multiplexity PCR Based on PCR Suppression
نویسندگان
چکیده
We present a protocol for effi cient amplifi cation of a large number of DNA targets using a single-tube polymerase chain reaction (PCR) based on PCR suppression. This method allows amplifi cation of each DNA target with only one target-specifi c primer whereas the other primer is common for all targets. Thus, this approach requires n+1 primers for n targets instead of 2n in conventional PCR and substantially reduces the cost and complexity of the assay. The method has been successfully applied for amplifi cation of 30 SNP-related DNA targets from human genomic DNA and for genotyping complex (plant) genomes.
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