Antioxidant effect of sodium selenite on thioacetamide-induced renal toxicity

Thioacetamide is an organosulfur compound and reasonably anticipated to be a human carcinogenic and toxic effect. Sodium selenite is considered to be an anticarcinogenic and antioxidant, therefore, the aim of study is to evaluate the toxic effect of thioacetamide and the role of sodium selenite on renal tissues by using rat model. 24 Albino wistar rats of male sex (b.w: 200±30gm) were divided into four groups (n = 6). Group I control (un treated), Group II received thioacetamide (200mg/kg b.w; i.p.) for 12 weeks; twice in a week, Group III received sodium selenite (1mg/kg b.w; i.p.) for 12 week; thrice in a week and Group IV received sodium selenite (1mg/kg: thrice in a week)+thioacetamide (200mg/kg: twice in a week) for 12 weeks (sodium selenite was given 30 minute prior to thioacetamide administration). Thioacetamide mediated toxic effects are measured in terms of renal functions; creatinine and urea, oxidative stress; MDA and antioxidant status; SOD, GSH and catalase levels. The significant increase in urea level and decrease GSH and MDA level in thioacetamide treated group was observed. Sodium selenite treatment restored the glutathione level in thioacetamide+sodium selenite treated group. The results describe decreased MDA levels which show the toxic effect of thioacetamide on renal tissues while the sodium selenite did not over come this toxic effect but explain its antioxidant property by increasing the GSH level in renal tissues.