A Study of Malachite Green Staining of Leprosy Bacilli.
نویسنده
چکیده
منابع مشابه
In vitro methods for determination of viability of mycobacteria: comparison of ATP content, morphological index and FDA-EB fluorescent staining in Mycobacterium leprae.
Bacilli were purified from the 23 cases of multi bacillary type of leprosy. The ATP content of these bacilli was assayed by a firefly bioluminescent technique which is capable of detecting a very small number of cultivable mycobacteria as assessed by colony counts. The A TP content was compared with morphological index (MI) and FDA-EB staining of bacilli from the same specimens. It was observed...
متن کاملA new method for staining leprosy bacilli.
To a paper on a new method for staining tubercle bacilli with Nachtblau which the writer published in 1941, Reenstierna added a short note (1), together with a plate, on its use in staining leprosy bacilli in nasal smears, films of blood, and sections of lepromata. As was the case with tubercle bacilli, the bacilli of leprosy were found to take a dark-blue color, to appear larger and more disti...
متن کاملChoosing the decolourizer and its strength to stain Mycobacterium leprae. Does it actually matter?
Leprosy bacilli are more easily decolourized during staining than tuberculosis bacilli, so a weaker concentration of decolourizer is usually recommended. In Indonesia, the same 'strong' decolourizer is used for identifying both organisms. In a study to compare the results using different concentrations of different decolourizers, no difference could be found in the bacterial index (BI). It is s...
متن کاملThe use of non-deparaffinized tissue sections for staining leprosy bacilli.
Reduced acid-fast staining of leprosy bacilli occurs during the dewaxing of paraffin sections by xylene and alcohols; the older and more decrepit bacilli being especially affected. By the use of non-deparaffinized sections, the leprosy bacilli which could not be stained with the usual carbol fuchsin are strongly stained. Moreover, non-deparafinized sections can be used for the periodic acid-car...
متن کاملThe effect of fixatives on staining procedures for lepra bacilli in tissues.
An essential to the study of the histiogenesis of leprosy consists of reliable demonstrations of the bacilli in tissues. In the older days, when celloidin embedding was more widely used, fair results were often seen. Paraffin embedding, however, has almost totally replaced celloidin in common practice, and the problem has become that of determining the conditions of fixation, dehydrating, and s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- International journal of Leprosy
دوره 32 شماره
صفحات -
تاریخ انتشار 1964