Molecular Detection of Bacterial Etiology of Rheumatoid Arthritis
Authors
Abstract:
Background and purpose: Etiology of rheumatoid arthritis is not fully recognized. The purpose of this study was to use universal and specific primers to trace bacteria in the blood and synovial fluid in patients with rheumatoid arthritis. Materials and methods: In this experimental study, a PCR method was developed to identify a wide range of bacteria in general and Staphylococcus aureus in specific. Ninety five synovial fluid and 100 blood samples being stored at -80°C were assayed. Genome extraction was performed. Universal primer pairs were used for amplification of 16SrRNA and a specific primer was used for nuc gene of Staphylococcus aureus. Then, the PCR product of the specific primer was sequenced and data were analyzed. Results: The samples of synovial fluid and blood samples of rheumatoid arthritis patients which were negative in bacteriological culture showed 33 (34%) and 36 (36%) cases to be positive for 16Sr RNA, respectively. Also, 21 cases of synovial fluid and only 1 blood sample were positive for Staphylococcus aureus. Conclusion: The results showed the presence of 16SrRNA gene of different bacteria, including Staphylococcus aureus in blood and synovial fluid of patients. Based on current findings, it is likely to explain a part of the etiology of rheumatoid arthritis, thereby modifying some treatment protocols.
similar resources
Control of Rheumatoid Arthritis in Developing Countries
بیماری روماتیسم قلبی یکی از شایعترین علل مرگ و میر ناشی از بیماریهای قلبی و عروقی در کشورهای در حال توسعه می باشد. براساس بررسیهای انجام شده، میزان شیوع بیماری در جامعه و مدارس از ۱ الی ۲۲ در هر هزار نفر کودک می باشد. تخمین زده میشود که تعداد مبتلایان به بیماریهای مزمن روماتیسم قلبی در کشورهای در حال توسعه ۳۰ میلیون و در کشورهای صنعتی ۱ / ۵ میلیون باشد. بسیاری از موارد مرگ و میر ناشی از این بیم...
full textEffects of Rheumatoid Arthritis on the Larynx
Introduction: The aim of the present study was to compare the videolaryngostroboscopic findings between patients with rheumatoid arthritis and vocally healthy controls. Materials and Methods: This case-control descriptive study was performed on 113 people, including 50 patients with rheumatoid arthritis and 63 controls. The participants were subjected to videolaryngost...
full textEtiology of Rheumatoid Arthritis: A Historical and Evidence-Based Perspective
Rheumatoid arthritis (RA) is a chronic, inflammatory, and destructive polyarthritis with numerous autoimmune features and the potential for extra-articular and systemic complications. Its etiology is still unknown but much progress has occurred in defining important mechanistic components of RA, leading to significant advances in its treatment. RA is a multifactorial and multistage disease, beg...
full textOral Bacteria Are Responsible for the Etiology of Rheumatoid Arthritis
A significant association has been shown to exist between periodontitis and rheumatoid arthritis (RA) in observational studies. Some intervention studies have suggested that periodontal treatment can reduce serum inflammatory biomarkers such as C-reactive protein, or erythrocyte sedimentation rate. Serum antibodies to oral bacteria have been found at increased levels in RA patients. In addition...
full textMy Resources
Journal title
volume 29 issue 173
pages 33- 39
publication date 2019-06
By following a journal you will be notified via email when a new issue of this journal is published.
No Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023