بررسی اثرات رازیانه رومی بر پدیده مهار منتشر شونده در بافت نئوکورتیکال مغز رت

Background and Aim: It is believed that cortical spreading depression (CSD) is underlying mechanism of aura and subsequent pain in migraine. This study was designed to investigate the effect of Pimpinella Anisum on CSD specifications in rat neocortical slices.  Materials and Methods: In this experimental study, CSD was induced in rat somatosensory cortical slices using two methods. At the first method, after KCl microinjection, the slices were washed out for 60 minutes with Pimpinella Anisum oil extract in different concentrations (0.01-50 μM/L). At the second method, slices were washed at artificial cerebrospinal fluid (ACSF) with low concentration of NaCl. Then, slices were washed with 50 μM/L Pimpinella Anisum oil extract for 60 minutes. In both methods, control cortical slices were washed in low alcohol containing CSF. Using extracellular potentials recording, the frequency, amplitude, duration, and propagation velocity of CSD was measured. All data were statistically analyzed.  Results: In KCl induced CSF model, the amplitude (P < 0.001) and duration (P < 0.02) of CSD was significantly decreased after superfusion of Pimpinella Anisum at higher concentrations with dose-dependent route. Nonetheless, adding Pimpinella Anisum insignificantly reduced the propagation velocity of CSD (P = 0.3). In CSD induction with low NaCl concentration ACSF model, adding Pimpinella Anisum to washing solution caused reduction of frequency (P = 0.03) and amplitude (P = 0.048) of CSD. However, the duration of CSD in this model showed no significant difference after using Pimpinella Anisum. Conclusion: Regarding to our results, theoretically, Pimpinella Anisum is able to suppress CSD phenomenon as underlying mechanism of migraine with aura.