Background: Bone Marrow Transplantation is one of the most important therapeutic methods in much malignant and nonmalignant disease. Patients with Bone Marrow Transplantation (BMT) following radiotherapy and chemotherapy will suffer from immuno-suppression. Therefore they are susceptible to get saprophytic fungi infection that sometimes are killer. Materials and Methods: The purpose of this cross-sectional survey is isolation of saprophytic fungi from patients with BMT and wards space and instruments. Therefore sampling from ventilator system (HEPA filter and common filter), air canal, air, hospital instruments and clinical samples (nasal discharge, sputum, urine) were done and cultured in sabouro dextrose agar with choloramphenicol (SC). In assessing total frequency from 4838 plates of wards space and instruments, 985 fungi colonies includes 21 genus were isolated.Results and Conclusion: Most fungi colonies present were Penicillium , Aspergillus and Cladosporium and low present were Trichoderma ,Stereptomyses, Chrysosporium, Rhizopus.
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