بررسی پلی مورفیسم ژن1 GSTM و سقط جنین در استان گیلان

Background and purpose: Spontaneous abortion (SA) is pregnancy termination prior to 20 weeks gestation or with a fetus born weighing less than 500 grams. The etiology of spontaneous abortion (SA) remains unclear, but it may be related to a possible genetic predisposition. The glutathione Stransferases (GSTs) are a family of enzymes involved in the detoxification of a wide range of chemicals. Polymorphic deletion in GSTM1 gene causes no enzyme production leading to no an antioxidant activity. In this study, the relationship between SA and GSTM1 gene polymorphism in Guilan province was investigated. Material and Methods: Genomic DNA was extracted from 80 patients with SA and 100 healthy women. PCR was performed in two stages of Internal Standard-Controlled PCR and Nested-PCR. Results: 37.5% of the cases with SA had the GSTM1 null genotype and 12% deletion was observed in the control group (P<0.002). The distribution of GSTM1-active genotypes was significantly different in patients and controls (P= 0.0001), as frequency of AA or AO (GSTM1) genotype in patients were 88% and in control group 15%. Conclusion: Homozygous deletion of the GSTM1 gene in women could be a risk factor for spontaneous abortion in Guilan province