جدا سازی HDL2 و HDL3 و مقایسه غلظت کلسترول آنها بین گروه شاهد و مبتلایان به آترواسکلروز

The major Lipoprotein classes are heterogenous. Human serum High-Density-Lipoproteins (HDL) are separated into two major subfractions (HDL2, d=1.063-1.125 g/ml, HDL3, d=1.125-1.210 g/ml) by a simple double precipitation method. There was a strong inverse relation between HDL-Cholesterol (HDL-C) and subfractions HDL2-C and HDL3-C with atherosclerosis. This study demonstrated that HDL-C (P<0.001) and both HDL2-C (P<0.001) and HDL3-C (P<0.01) levels are significantly lower (HDL-C=-35%, HDL2-C=-52%, and HDL3-C=-26%) in the atherosclerosis patients (Coronary-Heart-Disease) than normal subjects. This data supports the proposal that the latter surfation is the major contributor to the anti atherogenic role of serum HDL-C, HDL2-C and HDL3-C.