Analysis of KRAS gene mutation associated with Helicobacter pylori infection in patients with gastric cancer
Objective(s): KRAS proto-oncogene mutation can be considered a diagnostic factor for treating various malignancies. Helicobacter pylori infection, a risk factor for stomach cancer, may cause DNA damage and genetic changes. The aim of the current study was to assess the association of gastric cancer and KRAS mutation, demographic factors, and H. pylori infection.Materials and Methods: DNA was extracted from a total of 140 FFPE gastric cancer tissue samples. detection of KRAS mutation (codons 12 and 13) in tumors was performed by PCR amplification, followed by gel electrophoresis and DNA sequencing. PCR diagnosed any H. pylori infection.Results: KRAS mutation was detected in 6 of the 140 (4.2%) gastric cancer tissue samples. 18 samples (12.8%), all of which were male (P<0.05), tested positive for H. pylori infection. KRAS mutations were present in 22.2% (4/18) of the samples with H. pylori infection (P<0.05). The mean age of patients was 62.25±12.61 years (range: 30–93 years). A male predominance (2.5 to 1) was reported in the gastric cancers, and at diagnosis, women were significantly younger than men (P=0.004). No association was observed between age or gender and KRAS mutation. Neither was one found between age and H. pylori infection. Tumors from H. pylori+ subjects were significantly more likely to have KRAS mutation than tumors from H. pylori- subjects (OR=17.1).Conclusion: The data suggest that H. pylori infection when compared with the absence of H. pylori infection, is associated with a higher prevalence of KRAS mutation in gastric cancer.
Presence of cagA Gene in Patients with Gastric Cancer and Gastritis with Helicobacter pylori Infection
Background and purpose: Helicobacter pylori is a gram-negative, micro-aerophilic, and spiral-shaped bacillus. Infection with this bacterium can lead to gastritis, ulcers, and even gastric cancer. This study was conducted to evaluate the presence of the cagA gene among patients with gastritis and gastric cancer. Materials and methods: This study was performed in patients with gastritis (n=40) a...متن کامل
Helicobacter pylori (H. pylori) is a gram-negative microaerophilic bacterium that has been introduced as a cause of mucosal inflammation and gastric cancer. The most important pathogenic factors are VacA and CagA, which are associated with increased disease severity in clinical strains. Autophagy is a protected lysosomal degradation pathway degrading cytoplasmic content and is important in host...متن کامل
HELICOBACTER PYLORI Promoter methylation of E-cadherin gene in gastric mucosa associated with Helicobacter pylori infection and in gastric cancer
Background: E-cadherin is an adhesion molecule involved in tumour invasion/metastasis. Silencing of E-cadherin by promoter CpG methylation has been shown in both familial and sporadic gastric cancers. Helicobacter pylori is a class I carcinogen in gastric cancer. Aims: This study was undertaken to investigate the association between methylation of E-cadherin and H pylori in gastric mucosa from ...متن کامل
Gastric cancer is a world health problem and depicts the fourth leading mortality cause from malignancy in Mexico. Causation of gastric cancer is not only due to the combined effects of environmental factors and genetic variants. Recent molecular studies have transgressed a number of genes involved in gastric carcinogenesis. The aim of this review is to understand the recent basics of gene expr...متن کامل
Prevalence of Coinfection Helicobacter pylori with Non-Helicobacter pylori Helicobacters Species in Patients Suffering from Gastric Diseases in Iran
Introduction: In addition to Helicobacter pylori, non-Helicobacter pylori helicobacters (NHPHs) have been diagnosed in the humans stomach that caused gastrointestinal diseases. This study aimed to evaluate the coinfection of H. pylori with NHPHs species in patients with gastric disorders in Iran. Materials & Methods: This cross-sectional study was performed on 421 gastric biopsies form dysp...متن کامل
BACKGROUND Helicobacter pylori (H. pylori) is the most common chronic infectious agent in the stomach. Most importantly, it may lead to atrophy, metaplasia and cancer. The aim of this study was to investigate the incidence of H. pylori infection and to detect early mucosal changes that may lead to malignant degeneration in children. MATERIALS AND METHODS Children who underwent upper gastroint...متن کامل
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دوره 22 شماره 5
صفحات 529- 533
تاریخ انتشار 2019-05-01
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