Backcross breeding between TGGG hybrid grouper (Epinephelus fuscoguttatus E. lanceolatus) and giant grouper (E. lanceolatus)
This paper reports on the first successful backcross breeding between TGGG hybrid grouper and GG in captivity. To establish a seed production technique for this new backcross grouper, eggs and larval development were observed. Three successful spawning was observed in November 2018, September 2019 and November 2019. The eggs were collected using stripping methods and the sperms were collected using sperms collector. About 1.2 to 1.3 litres of eggs were collected in each spawning. Eggs and larval development of backcross TGGG hybrid and GG were almost similar to its parental species and other Epinephelus species. Fertilized eggs were measured 0.860 to 0.915 mm in diameter, with oil globule 0.190 to 0.214 mm in diameter. Fertilization and hatching rate were in between 80 to 90 %. Newly hatched larvae range from 1.64 to 1.99 mm and commenced feeding at 60 d AH. Larval dorsal and pelvic spines started to develop from 9 d AH and fins were formed on 21 d AH. By the age of 30 d AH, the fish entered juvenile stage. At 60 d AH, the fry’s body shape and colour resembled those of the adult. After more than 300 d AH, the fish reached 1 kg in weight. The backcross between female TGGG and male GG is expected to perform even better growth and survival rate than TGGG because the backcross carries more genetic material from the GG, which would be ideal for mass production in the aquaculture industry.
Susceptibility of farmed juvenile giant grouper Epinephelus lanceolatus to a newly isolated grouper iridovirus (genus Ranavirus).
A ranavirus was isolated from the diseased farmed groupers (Grouper iridovirus in genus Ranavirus, GIV-R), Epinephelus hybrids (blotchy rock cod, Epinephelus fuscoguttatus ♀×giant grouper, Epinephelus lanceolatus ♂), in Sanya, Hainan, in July 2013. In this study, susceptibility of farmed juvenile giant grouper E. lanceolatus to GIV-R was determined by intraperitoneally injection. The cumulative...متن کامل
Development of Pedigree Classification Using Microsatellite and Mitochondrial Markers for Giant Grouper Broodstock (Epinephelus lanceolatus) Management in Taiwan
Most giant groupers in the market are derived from inbred stock. Inbreeding can cause trait depression, compromising the animals' fitness and disease resistance, obligating farmers to apply increased amounts of drugs. In order to solve this problem, a pedigree classification method is needed. Here, microsatellite and mitochondrial DNA were used as genetic markers to analyze the genetic relation...متن کامل
Roe Protein Hydrolysates of Giant Grouper (Epinephelus lanceolatus) Inhibit Cell Proliferation of Oral Cancer Cells Involving Apoptosis and Oxidative Stress
Roe protein hydrolysates were reported to have antioxidant property but the anticancer effects were less addressed, especially for oral cancer. In this study, we firstly used the ultrafiltrated roe hydrolysates (URH) derived from giant grouper (Epinephelus lanceolatus) to evaluate the impact of URH on proliferation against oral cancer cells. We found that URH dose-responsively reduced cell viab...متن کامل
Molecular analysis of RNA1 and RNA2 sequences from a betanodavirus isolated from giant grouper (Epinephelus lanceolatus) in Australia
a Biosecurity Sciences Laboratory, Biosecurity Queensland, Queensland Department of Agriculture, and Fisheries, Block 12, Health and Food Sciences Precinct, 39 Kessels Road, Coopers Plains Queensland, 4108, PO Box 156, Archerfield BC, Brisbane, QLD, Australia b Department of Agriculture andWater Resources, Building 114, Catalina Crescent, Airport Business Centre, Cairns Airport, Box 96, AAC Cai...متن کامل
Sperm of the giant grouper: cryopreservation, physiological and morphological analysis and application in hybridizations with red-spotted grouper
In order to develop excellent germplasm resources for giant grouper (Epinephelus lanceolatus), cryopreservation of giant grouper sperm was examined in the present study. Firstly, 13 kinds of sperm dilution (ELS1-3, EM1-2, TS-2, MPRS, ELRS0-6) were prepared with physiological salt, sucrose, glucose and fetal bovine serum. The physiological parameters of ELRS3 (ratio of fast motion, ratio of slow...متن کامل
Comparative Transcriptomic Study of Muscle Provides New Insights into the Growth Superiority of a Novel Grouper Hybrid
Grouper (Epinephelus spp.) is a group of fish species with great economic importance in Asian countries. A novel hybrid grouper, generated by us and called the Hulong grouper (Hyb), has better growth performance than its parents, E. fuscoguttatus (Efu, ♀) and E. lanceolatus (Ela, ♂). We previously reported that the GH/IGF (growth hormone/insulin-like growth factor) system in the brain and liver...متن کامل
دوره 7 شماره 2
صفحات 49- 62
تاریخ انتشار 2021-02
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