Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran
Objective(s): Staphylococcus aureus can cause several infections. Its capability to form biofilm has been reported to be a vital property involved in the bacteria’s pathogenesis. Various genes contributing to biofilm formation have not yet been completely clarified. This study was designed to evaluate the factors influencing adherence and biofilm formation in S. aureus isolated from paediatric patients.Materials and Methods: One hundred and ninety-seven S. aureus isolates were obtained from pediatric patients and confirmed with phenotypic and molecular examinations. Antimicrobial susceptibility testing and biofilm formation were evaluated using standard methods. The genes encoding adhesion and virulence factors were investigated by the PCR method. Results: The most efficient antibiotics against S. aureus isolates were vancomycin and linezolid. Approximately, 54.2% of MSSA and 85.6% of MRSA isolates were biofilm producers according to the microtiter test. Our analysis indicated that MRSA isolates are better able to form biofilm compared with MSSA isolates. All isolates harbored clfA, fnbpA, icaA, icaB, icaC, and icaD, while clfB, fnbB, hlg, and pvl were detected in 99.5%, 42.1%, 97.5%, and 5.6% of isolates, respectively. In addition, a significant difference was found in fnhB gene and biofilm formation.Conclusion: Our findings showed a significant correlation between mecA and pvl genes and MRSA and biofilm formation in S. aureus isolates. Additionally, this study indicated the significant role of the fnhB gene as a major marker for S. aureus biofilm formation. Therefore, further experiments are warranted to exactly elucidate the function of the fnhB gene in the formation of biofilm.
Detection of Intracellular Adhesion (ica) and Biofilm Formation Genes in Staphylococcus aureus Isolates from Clinical Samples
Background: The nosocomial infections that cause the establishment of biofilms on the embedded biomedical surfaces are the leading cause of sepsis and are often related to colonization of implants by Staphylococcus epidermidis. Materials and Methods: A total of 40 clinical S. aureus isolates were collected from Zabol, Iran. The ability of these strains to form biofilm was determined by microli...متن کامل
Detection of icaABCD Genes and Biofilm Formation in Clinical Isolates of Methicillin Resistant Staphylococcus aureus
Background & Objectives: Methicillin resistance Staphylococcus aureus (MRSA) is one of the most important pathogens that causes several nosocomial and community infections. Adhesion to surfaces and biofilm formation is considered main step in staphylococcal infection. The aims of this study were to determine presence oficaABCD genes and relation to the biofilm formation in of...متن کامل
Biofilm Formation and Detection of IcaAB Genes in Clinical Isolates of Methicillin Resistant Staphylococcus aureus
Objective(s) Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial and community infections. Biofilm formation, mediated by a polysaccharide intercellular adhesin (PIA) and encoded by the ica operon, is considered to be an important virulence factor in both S. epidermidis and S. aureus. However, the clinical impact of the ica locus and PIA production is less w...متن کامل
Comparison of Biofilm Formation between Methicillin-Resistant and Methicillin-Susceptible Isolates of Staphylococcus aureus
Background: The aim of this study was to compare the biofilm formation and the prevalence of biofilm-associated genes between the isolates of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) Staphylococcus aureus. Methods: In total, 209 S. aureus isolates were collected. The antibiotic susceptibility test was conducted using nine antibiotics according to the guidelines of Clinica...متن کامل
Detection of icaABCD Genes in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus from Patients in Iran
Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen that causes several nosocomial or community-acquired infections. Adhesion to surfaces and subsequent biofilm formation are the major phases of a staphylococcal infection. The aim of this study was to detect the presence of icaABCD genes in clinical isolates of MRSA. Methods: A total of 110 clinical Staphyl...متن کامل
Screening of genes encoding adhesion factors and biofilm formation in Staphylococcus aureus isolates from poultry.
Staphylococcus aureus isolates recovered from poultry between 1970 and 1972 (90 old isolates) and in 2006 (81 recent isolates) were screened for the presence of bap, icaA and icaD genes associated with biofilm formation, and for bbp, cna, ebpS, eno, fib, fnbA, fnbB, clfA and clfB genes that encode microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). Most of the old iso...متن کامل
دوره 23 شماره 5
صفحات 691- 698
تاریخ انتشار 2020-05-01
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