Comparison of the efficacy of Piascledine and transforming growth factor β1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds

نویسندگان

  • Ali Valiani Department of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
  • Batul Hashemibeni Department of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
  • Farhad Golshan Iranpour Department of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
  • Maryam Aliakbari Department of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
  • Mohamad Kazemi Department of Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
  • Mojtaba Esmaeili Department of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده

Objective(s):The aim of this study was to compare the chondrogenic induction potential of Piascledine and TGF-β1 on adipose-derived stem cells (ADSCs) in fibrin and fibrin-alginate scaffolds.  Materials and Methods: Human subcutaneous adipose tissues were harvested from three patients who were scheduled to undergo liposuction. Isolated ADSCs were proliferated in a culture medium. Then, the cells were seeded in fibrin or fibrin-alginate scaffolds and cultured for 14 days in a chondrogenic medium containing Piascledine, TGF-β1, or both. The rate of cell proliferation and survival was evaluated by using MTT [3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide] assay and the rate of the expression of type II collagen, aggrecan, and type X collagen genes was evaluated by real-time polymerase chain reaction (real-time PCR) method. Results: The MTT results showed that Piascledine is able to enhance the proliferation and survival of ADSCs in fibrin scaffolds in comparison to other groups (P

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Comparison of the efficacy of piascledine and transforming growth factor β1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds

Objectives The aim of this study was to compare the chondrogenic induction potential of Piascledine and TGF-β1 on adipose-derived stem cells (ADSCs) in fibrin and fibrin-alginate scaffolds. Materials and Methods Human subcutaneous adipose tissues were harvested from three patients who were scheduled to undergo liposuction. Isolated ADSCs were proliferated in a culture medium. Then, the cells ...

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عنوان ژورنال:

دوره 21  شماره 2

صفحات  212- 218

تاریخ انتشار 2018-02-01

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