Effect of Berberis vulgaris Aqueous Extract on the Apoptosis, Sodium and Potassium in Hepatocarcinogenic Rats

Objective(s) The effect of Berberis vulgaris aqueous extract in hepatocarcinogenic rats was studied to investigate the apoptotic and sodium, potassium elements properties. A loss of both intracellular potassium and sodiumoccurs when apoptotic cells shrink and prior to the loss of membraneintegrity. Materials and Methods Forty-eight Sprague dawley rats were randomly divided into 2 groups, normal and cancerous. Each group was divided into 4 subgroups. The first subgroup acted as normal control while the others were treated with 25, 50 and 100 mg/kg of Berberis vulgaris extract (BVE) and respectively considered as NC, NC25, NC50 and NC100. The first subgroup of cancerous rats acted as cancer control while the others were treated with 25, 50 and 100 mg/kg of BVE and considered as C, C25, C50 and C100. Ion selective electrode (ISE) method was used to measure the level of sodium, potassium, and chloride. TUNEL assay used for the detection of apoptosis cells. Results Microscopic observations of the TUNEL-positive apoptotic cells revealed a significant difference (P