Effect of magnetic fluid hyperthermia with dendrimer coated iron oxide nanoparticles on breast cancer in BALB/c mice
Introduction: Magnetic fluid hyperthermia (MFH) is a promising therapeutic method in cancer therapy with using magnetic nanoparticles (NPs). In this study, we assessed the effect of MFH on mechanisms of cell death in murine breast cancer cell line (MC4-L2) and also the treatment of breast tumor in BALB/C mice using four generation dendrimer coated iron oxide nanoparticles (G4@IONPs). Materials and Methods: Immediately after MFH, the viability of cells was assessed in all groups (MFH+NPs, only MFH, only NPs, control) by MTT assay. In order to determine the number of apoptosis and mRNA copies for Bax and Bcl-2 in the cells after MFH, TUNEL assay and RT-PCR were performed, respectively. The mice were exposed three times during twenty minutes to an MFH (12 kA/m, 300 kHz) after intertumoral injection of 5mg G4@IONPs. The temperature during MFH was monitored with an infrared thermometer (FLIR Systems). Tumor volume was measured during 28 days after MFH. Histopathology, Immunohistochemical staining, and TUNEL assay were performed in the liver and tumor tissues. Results: Cell viability percentage in the group of MFH+NPs decreased significantly (33%, P<0.05) while the viability in other groups had no any significant change. Also, the number of apoptotic cells and Bax/Bcl-2 ratio in MFH+NPs increased significantly. The MFH led to significantly reduce the volume of the tumor 30 days after the treatment compared with control mice (saline injection) (p < 0.05). tissue destruction obviously was seen in tumor histopathology of MFH+NPs mice. CD31 as angiogenesis marker decreased in treated tumors compared with control ones and furthermore, apoptosis significantly increased in treated tumor tissues. Conclusion: Our study showed MFH with G4@IONPs could demolish the cancer cells mainly because of increasing the apoptosis and regulating the apoptotic genomes including Bax and Bcl-2. MFH can applied as a clinical anti-cancer strategy due to inhibition of tumor angiogenesis.
Introduction: Recently, some studies have focused on dendrimer nanopolymers as an MRI contrast agent or a vehicle for gene and drug delivery. Considering the suitable properties of these materials, they are appropriate candidates for coating iron oxide nanoparticles which are applied to magnetic hyperthermia. To the best of our knowledge, the novelty of this study is the inves...متن کامل
An investigation of the effect of hyperthermia using iron and magnetic nanoparticles in cancer treatment
Introduction: hyperthermia using different methods such as microwave and magnetic waves is one of the methods to treat cancer. In this method, iron and magnetic nanoparticles are used to increase the temperature and increase the effect of hyperthermia as auxiliary treatment with chemotherapy and radiotherapy. In this study, the role of iron and magnetic nanoparticles have been ...متن کامل
Hydroxyapatite Coated Iron Oxide Nanoparticles: A Promising Nanomaterial for Magnetic Hyperthermia Cancer Treatment
Targeting cancer cells without injuring normal cells is the prime objective in treatment of cancer. In this present study, solvothermal and wet chemical precipitation techniques were employed to synthesize iron oxide (IO), hydroxyapatite (HAp), and hydroxyapatite coated iron oxide (IO-HAp) nanoparticles for magnetic hyperthermia mediated cancer therapy. The synthesized well dispersed spherical ...متن کامل
Biodistribution, pharmacokinetics, and toxicity of dendrimer-coated iron oxide nanoparticles in BALB/c mice
Background The possibility of using a specific nanoparticle in nanomedicine highly depends on its biodistribution profile and biocompatibility. Due to growing demand for iron oxide nanoparticles (IONPs) and dendrimers in biomedical applications, this study was performed to assess the biodistribution, pharmacokinetics, and toxicity of dendrimer-coated iron oxide nanoparticles (G4@IONPs). Mater...متن کامل
Background & Aims: In spite of frequent produce and use of magnetic nanoparticles in biological fields, there are few studies on their side effects, especially under in-vivo conditions. Method: In this research, the effect of the single-dose intraperitoneal injection of DMSA (dimercaptosuccinic acid) coated magnetic iron oxide nanoparticles (Fe3O4) in different doses (50, 100, 200 and 300 mg/kg...متن کامل
Using the thermal decomposition of organometallics method we have synthesized high-quality, iron oxide nanoparticles of tailorable size up to ~15nm and transferred them to a water phase by coating with a biocompatible polymer. The magnetic behavior of these particles was measured and fit to a log-normal distribution using the Chantrell method and their polydispersity was confirmed to be very na...متن کامل
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دوره 15 شماره Special Issue-12th. Iranian Congress of Medical Physics
صفحات 127- 127
تاریخ انتشار 2018-12-01
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