Effects of seed inoculation by cycocel and biofertilizers on yield, antioxidant enzymes activity and some biochemical traits of wheat (Triticum aestivum L.) under soil salinity condition

In order to study the effects of seed inoculation by cycocel and biofertilizers on yield, antioxidant enzymes activity and some biochemical traits of wheat (Triticum aestivum L.) under soil salinity condition, a factorial experiment was conducted based on randomized complete block design with three replications in a research greenhouse of Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili in 2016. Treatments were included salinity in four levels [no salt (as control), salinity 25, 50 and 75 mM by NaCl and seed inoculation by biofertilizers and cycocel in six levels [no inoculation with cycocel and bio fertilizers as control, inoculation by Pseudomonas, Azosprilium, both inoculation Azosprilium and Pseudomonas, inoculation by cycocel in concentration 10-5 and 10-6 mM]. The results showed that 75 mM salinity increased protein content and antioxidant enzymes activity such as catalase, peroxidase and polyphenol oxidase (43.39, 103.9, 97.83 and 143 % respectively) in comparison with no salinity. The lowest electrical conductivity (65.8 µs.cm-1) and the highest relative water content in flowering stage (87.86 %), protein content (17.7%) and grain yield (2.83 g per plant) were obtained in no salt and both inoculation Azosprilium and Pseudomonas. The highest electrical conductivity (111.83 µs.cm-1)  and the lowest relative water content in grain filling period (49.6 %), protein content (10.57 %) and grain yield (0.98 g per plant) were obtained in the highest salinity level and no inoculation with cycocel and bio fertilizers.