Genetic properties of blaCTX-M and blaPER β-lactamase genes in clinical isolates of Enterobacteriaceae by polymerase chain reaction
Objective(s):blaCTX-Mand blaPER are two genes that encode class A extended-spectrum β-lactamases (ESBLs) and can be responsible for therapeutic problems. This study was carried out to evaluate the molecular properties of these genes in clinical isolates of Enterobacteriaceae by polymerase chain reaction (PCR), restriction digestion and sequencing. Materials and Methods: During six months, starting from January 2012, one hundred clinical isolates of Enterobacteriaceae were collected from urinary samples. The ESBL-producing isolates were detected by phenotypic confirmation test. After plasmid extraction, blaPER and blaCTX-M genes were detected using PCR by specific primers. The blaCTX-M PCR products were digested with Taq1, and two of the blaCTX-Mgenes were sequenced. Results: Phenotypic tests showed that 27 (27%) isolates were ESBL producers with the highest frequency for Klebsiella pneumoniae (47.4%) andEscherichia coli (17.9%). Twenty six (26%) of Enterobacteriaceae isolates harbored the blaCTX-Mgene, and none of them had blaPER. The restriction analysis of PCR products showed that all blaCTX-M amplified products had the same patterns. Both sequenced bacteria were CTX-M-15 type ESBL carriers. Conclusion: The results of this study showed the blaCTX-M-15gene in Enterobacteriaceae isolates for the first time in Mashhad, Iran. High degrees of associated resistance to co-trimoxazole and gentamicin were found in ESBL producers. Therefore, an integrated and regular management of antibiotic prescription need to be trained in our society.
genetic properties of blactx-m and blaper β-lactamase genes in clinical isolates of enterobacteriaceae by polymerase chain reaction
objective(s):blactx-mand blaper are two genes that encode class a extended-spectrum β-lactamases (esbls) and can be responsible for therapeutic problems. this study was carried out to evaluate the molecular properties of these genes in clinical isolates of enterobacteriaceae by polymerase chain reaction (pcr), restriction digestion and sequencing. materials and methods: during six months, start...متن کامل
Methods: A total of 642 clinical isolates were collected consecutively during a 4 month period (September to December 2004). Resistance or reduced susceptibility to cefotaxime and/or ceftazidime and/or aztreonam was assessed using double disc synergy tests using clavulanic acid, cefotaxime, ceftazidime and aztreonam to identify putative ESBL-producing isolates. The ESBL determinants were charac...متن کامل
Detection of Nocardia Asteroides Complex in Clinical Isolates by Real-Time Polymerase Chain Reaction
Background and Aims: Nocardia asteroides complex is the most common cause of infectious diseases due to nocardiosis. Interspecies differentiation of Nocardia genera is essential for prognosis and timely proper treatment, as well as for epidemiological studies. Since each genus has its own antibiotic resistance, precise careful diagnosis is of prime importance. As compared to biochemical and phe...متن کامل
Prevalence of blaCTX-M1, blaCTX-M14 and blaCTX-M15 Genes among Escherichia ColiIsolated from Urinary Tract Infections in Outpatients, Kermanshah City, Iran
Background and Aims: The rate of urinary tract infection caused by the extended-spectrum-β-lactamase- (ESBL) producing Escherichia coli (E. coli) is increasing worldwide. This study aimed to assess the frequency of blaCTX-M genes in the E. coli isolated from urinary tract infection (UTI) in outpatients. Methods: 240 E. coli bacteria were isolated from the outpatients’ urine samples in the Kerma...متن کامل
Evaluation of the β-Lactamase Disk Test Method in the Detection of Extended-Spectrum-β- Lactamases in Clinical Isolates of Multidrug-Resistant Pseudomonas aeruginosa
Background & Aims: The production of extended-spectrum-β-lactamases (ESBLs) is the main mechanism of resistance to β-lactam antibiotics. The outbreak of isolates simultaneously possessing several resistance mechanisms to β-lactam antibiotics caused a decrease in sensitivity of the confirmatory tests for ESBL. The aim of this study was the evaluation of the β-lactamase disk test method in the de...متن کامل
دوره 17 شماره 5
صفحات 378- 383
تاریخ انتشار 2014-05-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.