Increased Litter Size and Suckling Intensity Stimulate mRNA of RFamide-related Peptide in Rats

نویسندگان

  • Ali Moghadam Biotechnology Institute, College of Agriculture, Shiraz University, Shiraz, Iran
  • Ali Niazi Biotechnology Institute, College of Agriculture, Shiraz University, Shiraz, Iran
  • Amin Tamadon Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Atefeh Noroozi Department of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, Iran
  • Mohammad Reza Jafarzadeh Shirazi Department of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, Iran
چکیده مقاله:

Background RFamide-related peptide-3 (RFRP-3) inhibits gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in rats. This study evaluates the effects of litter size and suckling intensity on RFRP mRNA expression in the dorsomedial hypothalamic nucleus (DMH) of rats. MaterialsAndMethods A total of 32 pregnant and 4 non-lactating ovariectomized (control group) Sprague-Dawley rats were used in this experimental study. Lactating rats were allotted to 8 equal groups. In 3 groups, the litter size was adjusted to 5, 10, or 15 pups upon parturition. Dams were allowed to suckle their pups continuously until 8 days postpartum. In the other 3 groups, the litter size was adjusted to 5 pups following birth. These pups were separated from the dams for 6 hours on day 8 postpartum, after which the pups were allowed to suckle for 2.5, 5, or 7.5 minutes prior to killing the dams. In 2 groups, lactating rats with 10 and 15 pups were separated from their pups for 6 hours on day 8 postpartum. In these groups, the pups were allowed to suckle their dams for 5 minutes before the dams were killed. All rats were killed on day 8 postpartum and the DMH was removed from each rat. We evaluated RFRP mRNA expression using realtime polymerase chain reaction (PCR). Results The expression of RFRP mRNA in the DMH increased with increased litter size and suckling intensity compared to the controls. The effect of suckling intensity on the expression of RFRP mRNA was more pronounced compared to the litter size. Conclusion Increased litter size and suckling intensity stimulated RFRP mRNA expression in the DMH which might contribute to lactation anestrus in rats.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

increased litter size and suckling intensity stimulate mrna of rfamide-related peptide in rats

background: rfamide-related peptide-3 (rfrp-3) inhibits gonadotropin releasing hormone (gnrh) and luteinizing hormone (lh) secretion in rats. this study evaluates the effects of litter size and suckling intensity on rfrp mrna expression in the dorsomedial hypothalamic nucleus (dmh) of rats. materials and methods: a total of 32 pregnant and 4 non-lactating ovariectomized (control group) sprague-...

متن کامل

Increased Litter Size and Suckling Intensity Stimulate mRNA of RFamide-related Peptide in Rats

BACKGROUND RFamide-related peptide-3 (RFRP-3) inhibits gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in rats. This study evaluates the effects of litter size and suckling intensity on RFRP mRNA expression in the dorsomedial hypothalamic nucleus (DMH) of rats. MATERIALS AND METHODS A total of 32 pregnant and 4 non-lactating ovariectomized (control group) Sprague-...

متن کامل

Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus

Objective(s): The effect of litter size and suckling intensity on the expression of KiSS-1 mRNA in the arcuate nucleus (ARC) of rats were evaluated. Materials and Methods: Thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. Lactating rats were allotted to eight equal groups. In three groups, litter size was adjusted to 5, 10, or 15 pup...

متن کامل

increased litter size and suckling intensity inhibit kiss-1 mrna expression in rat arcuate nucleus

objective(s): the effect of litter size and suckling intensity on the expression of kiss-1 mrna in the arcuate nucleus (arc) of rats were evaluated. materials and methods: thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. lactating rats were allotted to eight equal groups. in three groups, litter size was adjusted to 5, 10, or 15 pup...

متن کامل

Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus

OBJECTIVES The effect of litter size and suckling intensity on the expression of KiSS-1 mRNA in the arcuate nucleus (ARC) of rats were evaluated. MATERIALS AND METHODS Thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. Lactating rats were allotted to eight equal groups. In three groups, litter size was adjusted to 5, 10, or 15 pups ...

متن کامل

P-222: Expression of RFamide-Related Peptide-3 and KiSS-1 mRNA in The Hypothalamus of Pregnant Rat

Background: There is no follicular development during pregnancy in the rat comparable to the changes of the estrous cycle. RFamide-related peptide-3 (RFRP-3) and kisspeptin were recognized as regulator of gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in several species, including the rat. The aim of the present study was to evaluate the relative expression of RFRP...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 9  شماره 3

صفحات  380- 386

تاریخ انتشار 2015-10-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023