Low-frequency vibration treatment of bone marrow stromal cells induces bone repair in vivo

نویسندگان

  • Chuanxiu Sun Department of Orthopedics, Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, 116031, P.R. China
  • Guangyu Du Department of Orthopedics, Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, 116031, P.R. China
  • Lidong Mi Department of Orthopedics, Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, 116031, P.R. China
  • Lu Zhang Department of Orthopedics, Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, 116031, P.R. China
  • Shengwei He Department of Orthopedics, Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, 116031, P.R. China
  • Wenzhi Zhao Department of Orthopedics, Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, 116031, P.R. China
  • Xuegang Sun Department of Orthopedics, Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, 116031, P.R. China
چکیده

Objective(s):To study the effect of low-frequency vibration on bone marrow stromal cell differentiation and potential bone repair in vivo. Materials and Methods:Forty New Zealand rabbits were randomly divided into five groups with eight rabbits in each group. For each group, bone defects were generated in the left humerus of four rabbits, and in the right humerus of the other four rabbits. To test differentiation, bones were isolated and demineralized, supplemented with bone marrow stromal cells, and implanted into humerus bone defects. Varying frequencies of vibration (0, 12.5, 25, 50, and 100 Hz) were applied to each group for 30 min each day for four weeks. When the bone defects integrated, they were then removed for histological examination. mRNA transcript levels of runt-related transcription factor 2, osteoprotegerin, receptor activator of nuclear factor k-B ligan, and pre-collagen type 1 a were measured. Results:Humeri implanted with bone marrow stromal cells displayed elevated callus levels and wider, more prevalent, and denser trabeculae following treatment at 25 and 50 Hz. The mRNA levels of runt-related transcription factor 2, osteoprotegerin, receptor activator of nuclear factor k-B ligand, and pre-collagen type 1 a were also markedly higher following 25 and 50 Hz treatment. Conclusion:Low frequency (25–50 Hz) vibration in vivo can promote bone marrow stromal cell differentiation and repair bone injury.

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low-frequency vibration treatment of bone marrow stromal cells induces bone repair in vivo

objective(s):to study the effect of low-frequency vibration on bone marrow stromal cell differentiation and potential bone repair in vivo. materials and methods:forty new zealand rabbits were randomly divided into five groups with eight rabbits in each group. for each group, bone defects were generated in the left humerus of four rabbits, and in the right humerus of the other four rabbits. to t...

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عنوان ژورنال:

دوره 20  شماره 1

صفحات  23- 28

تاریخ انتشار 2017-01-01

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