Mesobuthus eupeus venom induced injury in the colorectal carcinoma cell line (HT29) through altering the mitochondria membrane stability

نویسندگان

  • Atefeh Raesi Vanani Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran|Department of Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  • Layasadat Khorsandi Cell & Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran|Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  • Leila Zeidooni Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  • Masoud Mahdavinia Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran|Department of Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  • Massood Valizade Cell & Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran|Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  • Mohsen Rezaei Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran|Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
چکیده

Objective(s): The purpose of this study was to investigate cytotoxicity and membrane toxicity effects induced by Mesobuthus eupeus venom (MEV) on the HT-29 cell line. Materials and Methods: To determine the in vitro cytotoxicity via MTT assays, HT-29 (as cancer cell line) and Hek-293T (as normal cell) were treated through different concentrations of MEV, and cytotoxicity effects were then measured through assessment of mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS) generation, and apoptosis induction. The colony formation assay was performed to measure the antiproliferative effect of MEV on HT-29 cells. Nuclei alterations were also observed during apoptosis following DAPI staining. Besides, atomic force microscopy (AFM) was used to detect alterations in morphology and ultrastructure of the cells at a nanoscale level.Results: According to MTT and clonogenic assays, MEV caused a significant decrease in cell viability and proliferation of HT-29 cells while it did not have any impact on normal cells and the IC50 value was found to be 10 µg/ml. Induction of apoptosis was also confirmed by flowcytometric analysis in HT-29 cells. Moreover, the results indicated that MEV had led to a suppression of proliferation and induction of apoptosis through increased ROS and depolarization of mitochondria. Furthermore, AFM imaging demonstrated apoptosis cell death after being treated with MEV in HT-29 cells. Conclusion: This study showed that MEV had an antiproliferative effect on HT-29 cells by inducing apoptosis through the mitochondria signaling pathway. These findings suggested that MEV could be used as a promising natural remedy for cancer treatment.

برای دانلود باید عضویت طلایی داشته باشید

برای دسترسی به متن کامل این مقاله و 10 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

gamma tocopherol and lovastatin additively induced apoptosis in human colorectal carcinoma cell line (ht29)

background programmed cell death (apoptosis) is a physiological process needed to remove unwanted or damaged cells. it has been hypothesized that any failure of programmed cell death leads to the development of neoplasm. identifying new agents which induce apoptosis in tumor cells is of great significance in treatment of neo­plasms. numerous studies suggest that exposure of tumor cells to stati...

متن کامل

First transcriptome analysis of Iranian scorpion, Mesobuthus eupeus venom gland

Scorpions are generally an important source of bioactive components, including toxins and other small peptides as attractive molecules for new drug development. Mesobuthus eupeus, from medically important and widely distributed Buthidae family, is the most abundant species in Iran. Researchers are interesting on the gland of this scorpion due to the complexity of its venom. Here, we have analyz...

متن کامل

Study on anti inflammatory effect of scorpion (Mesobuthus eupeus) venom in adjuvant-induced arthritis in rats

Rheumatoid arthritis is an autoimmune disease that causes chronic inflammation of the joints as well as other organs in the body. Adjuvant-induced arthritis models in inbred rats serve as relevant models for RA, having many clinical similarities to this disease. Using honey bee venom as a treatment for Rheumatoid arthritis is an ancient therapy in various parts of the world. However scorpion ve...

متن کامل

Purification of the Immunogenic Fractions and Determination of Toxicity in Mesobuthus eupeus (Scorpionida: Buthidae) Venom

BACKGROUND Scorpions stings are a health problem in many parts of the world. Mesobuthus eupeus (Buthidae) is the most prevalent species in the Middle East and Central Asia. Definition of toxicogenic and immunogenic characteristics of the venom is necessary to produce antidote. In this study, the noted properties of M. eupeus venom were evaluated. METHODS Venom was obtained by milking M. eupeu...

متن کامل

Oral Absorption of Mesobuthus eupeus Scorpion Venom in Mice

Background: To explore the oral absorption of scorpion venom an ELISA were designed in this study. Scorpions and their venom were been used for centuries as medical treatments in traditional medicine. The oral administration of drug referred as the convenient way, as there was not any publication about gastro-intestinal absorption of scorpion venom; this experiment checked oral absorption of Me...

متن کامل

Biochemical and Histopathological study of Mesobuthus eupeus scorpion venom in the experimental rabbits

In tropical and subtropical countries, envenomation by scorpions (so-called scorpionism) represents a serious public health problem. In the present study, the toxic effects of mice LD50 injections of Mesobuthus eupeus (Me) venom on the kidney and liver of anesthetized rabbits were investigated. Six rabbits were selected and ALT, AST, BUN and creatinine were measured at 0, 1 and 3 hours after...

متن کامل

ذخیره در منابع من

ذخیره در منابع من ذخیره شده در منابع من

{@ msg_add @}

  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی راحت تر خواهید کرد

دانلود متن کامل

برای دسترسی به متن کامل این مقاله و 10 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید


عنوان ژورنال:

دوره 23  شماره 6

صفحات  760- 767

تاریخ انتشار 2020-06-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2021