× خانه ژورنال ها پست ها ثبت نام ورود

Minocycline through attenuation of oxidative stress and inflammatory response reduces the neuropathic pain in a rat model of chronic constriction injury

نویسندگان

  • Amin Hasanvand 2Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran|Department of Pharmacology and Toxicology, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Amir Abbasnezhad Nutritional Health Research Center, Department of Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Asghar Aaliehpour Department of Pathology, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Hossein Amini-Khoyi Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
  • Razieh Choghakhori Nutritional Health Research Center, Department of Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Saeideh Darabi 2Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran|Department of Pharmacology and Toxicology, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran

چکیده

Objective(s): Several lines of evidence showed that minocycline possesses antioxidant and anti-inflammatory properties. This study aimed to demonstrate the effects of minocycline in rats subjected to chronic constriction injury (CCI). Materials and Methods: In this study four groups (n = 6–8) of rats were used as follows: Sham, CCI, CCI + minocycline (MIN) 10 mg/Kg (IP) and CCI + MIN 30 mg/Kg (IP). On days 3, 7, 14, and 21 post-surgery hot-plate, acetone, and von Frey tests were carried out. Finally, Motor Nerve Conduction Velocity Evaluation (MNCV) assessment was performed and spinal cords were harvested in order to measure tissue concentrations of TNF_α, IL-1β, Glutathione peroxidase (GPx), Superoxide dismutase (SOD) and Malondialdehyde (MDA). Extent of perineural inflammation and damage around the sciatic nerve was histopathologically evaluated. Results: Our results demonstrated that CCI significantly caused hyperalgesia and allodynia twenty-one days after CCI. MIN attenuated heat hyperalgesia, cold and mechanical allodynia and MNCV in animals. MIN also decreased the levels of TNF_α and IL-1β. Antioxidative enzymes (SOD, MDA, and GPx) were restored following MIN treatment. Our findings showed that MIN decreased perineural inflammation around the sciatic nerve. According to the results, the neuropathic pain reduced in the CCI hyperalgesia model using 30 mg/kg of minocycline. Conclusion: It is suggested that antinociceptive effects of minocycline might be mediated through the inhibition of inflammatory response and attenuation of oxidative stress.

برای دسترسی به متن کامل این مقاله و 10 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

ورود

منابع مشابه

Despite extensive investigations, the main mechanisms underlying neuropathic pain development are still not fully understood and there is no effective treatment for that. So intensive research is being done for finding new, efficient analgesic drugs. 56 male Wistar rats (230±30) were divided into 7 groups (n=8); control, sham, CCI and 3 Umbelliprenin groups (25, 50 and 100 microgram/rat)...

Introduction: Peripheral and central nerve injuries cause chronic neuropathic pain in many injured people besides motor disability. Exercise, as a behavioral and non-pharmacological treatment, has beneficial effects on people’s general health both for healthy and sick people. Therefore, this study was conducted to examine the effects of exercise on neuropathic pain induced by chronic cons...

Neuropathic pain involves injury or alteration of the normal sensory and modulatory nervous systems to produce a set of symptoms that are often difficult to treat. Previous study indicates that saffron has anti-inflammatory properties that may be mediate by neurotransmitter system. In this study we determine the role of cannabinoids receptors in peripheral and central effects of Crocin on behav...

Neuropathic pain is caused by a lesion or disease affecting the nervous systems, and is generally manifested as spontaneous pain, hyperalgesia, and allodynia. Previous study indicate that saffron has anti-inflammatory and antioxidant properties, we investigated whether saffron and crocin, a major constitute of saffron, would infleuence on behavioral responses of pain induced by chronic constric...

This study was designed to investigate the therapeutic effects of saffron (Crocus Sativus L) and its main constituent crocin on neuropathic pain behavioral responses induced by chronic constriction injury (CCI) in rats. Adult male Wistar rats (200 to 250 g) were randomly assigned into 5 groups: Sham + saline, CCI + saline, CCI+ saffron (30 mg/kg), CCI +crocin (15 mg/kg) and CCI + crocin (30 mg/...