miR-451 Up-regulation, Induce Erythroid Differentiation of CD133+cells Independent of Cytokine Cocktails

نویسندگان

  • Fatemeh Kouhkan Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
  • Majid Mossahebi-mohammadi Department of Clinical biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Masoud Soleimani Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  • Mehdi Mokhtari Departments of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Mehrdad Behmanesh Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
  • Morteza Daliri National institute of genetic engineering and biotechnology, Tehran, Iran
  • naser Mobarra Department of Clinical biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Reyhaneh Lahmy Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
  • Shahin Mohammadi Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran
چکیده

  Objective(s): Erythropoiesis is regulated by some extrinsic and intrinsic factors as microRNAs (miRNAs). miRNAs are endogenously small non-coding regulatory RNAs which play vital roles in the variety of cellular fate, critical processes; growth, apoptosis, metabolism, survival of the cells and specially differentiation. Several miRNAs such as miR-16 and miR-451 have been shown to be correlated with erythroid differentiation. Taking into account the importance of miRNAs in cellular differentiation, the goal of the present study was to examine the role of miRNAs in hematopoietic stem cells (HSC) differentiation into the erythroid cells in the absence of growth factors and stimulatory cytokines.   Materials and Methods: CD133+ stem cells were infected with lentiviruses containing miR-451/miR-16 precursor sequence, erythroid differentiation was evaluated using RT-PCR for hemoglobin chains and surface antigens, also by banzidine staining. Results: MiR-451up-regulation, but not miR-16, could induce α, β and γ-globin expression in CD133+ cells and have strong correlation with appearance of CD71 and CD235a markers in these cells. Moreover, miR-451 up-regulation increases the banzidine positive cells to ~ %40. Conclusion: Our results provide strong evidence that miR-451 up-regulation strongly induces erythroid differentiation and maturation of CD133+ stem cells. Hence, this method may provide a useful technique for the production of artificial blood RBC and be used as a new strategy for gene therapy of hemoglobinopathies, such as β-thalassemias and sickle cell anemia.

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mir-451 up-regulation, induce erythroid differentiation of cd133+cells independent of cytokine cocktails

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عنوان ژورنال:

دوره 16  شماره 6

صفحات  756- 762

تاریخ انتشار 2013-06-01

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